Role of Pneumococcal NanA Neuraminidase Activity in Peripheral Blood.

The most frequent form of hemolytic-uremic syndrome (HUS) is associated with infections caused by Shiga-like toxin-producing Enterohaemorrhagic (STEC). In rarer cases HUS can be triggered by . While production of Shiga-like toxins explains STEC-HUS, the mechanisms of pneumococcal HUS are less well-known produces neuraminidases with activity against cell surface sialic acids that are critical for factor H-mediated complement regulation on cells and platelets. The aim of this study was to find out whether neuraminidase NanA could trigger complement activation and hemolysis in whole blood. We studied clinical isolates and two laboratory strains, a wild-type strain expressing NanA, and a NanA deletion mutant for their ability to remove sialic acids from various human cells and platelets. Red blood cell lysis and activation of complement was measured by incubating whole blood with bacterial culture supernatants. We show here that NanA expressing strains and isolates are able to remove sialic acids from cells, and platelets. Removal of sialic acids by NanA increased complement activity in whole blood, while absence of NanA blocked complement triggering and hemolytic activity indicating that removal of sialic acids by NanA could potentially trigger pHUS.