一项关于通过酶联免疫吸附测定法（ELISA）和双向扩散法进行颚口线虫病免疫诊断的研究，特别提及了多氏颚口线虫的抗原性。

A study on immunodiagnosis of gnathostomiasis by ELISA and double diffusion with special reference to the antigenicity of Gnathostoma doloresi.

作者信息

Tada I, Araki T, Matsuda H, Araki K, Akahane H, Mimori T

机构信息

Department of Parasitic Diseases, Kumamoto University Medical School, Japan.

出版信息

Southeast Asian J Trop Med Public Health. 1987 Dec;18(4):444-8.

PMID:3129795

Abstract

In order to diagnose gnathostomiasis immunologically, Gnathostoma doloresi was evaluated for the antigenicity in comparison with G. hispidum which was recently reported in Japan by using micro-ELISA. The study revealed that G. doloresi can be used as the alternate source of antigen in the test. A significant increase of specific IgG antibodies was seen in 22 (73.3%) out of 30 gnathostomiasis cases. Although double diffusion was slightly less sensitive than ELISA, it was considered more specific than the latter method.

摘要

为了从免疫学角度诊断颚口线虫病，通过微量酶联免疫吸附测定法（micro-ELISA），将杜氏颚口线虫与日本最近报道的刚刺颚口线虫进行抗原性比较评估。研究表明，杜氏颚口线虫可作为该检测中抗原的替代来源。30例颚口线虫病病例中有22例（73.3%）出现特异性IgG抗体显著增加。虽然双向扩散法的敏感性略低于酶联免疫吸附测定法，但被认为比后者更具特异性。