Graham Kathleen L, McCowan Christina I, Caruso Kelly, Billson F Mark, Whittaker Cameron J G, White Andrew
Clinical Ophthalmology and Eye Health, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
University of Melbourne Veterinary Hospital, University of Melbourne, Melbourne, Victoria, Australia.
Vet Ophthalmol. 2020 Jan;23(1):97-112. doi: 10.1111/vop.12694. Epub 2019 Jul 12.
To evaluate the retina and optic nerve head (ONH) in canine eyes predisposed to glaucoma using optical coherence tomography (OCT).
Twenty-five eyes (24 dogs).
Measures of peripapillary retinal, retinal nerve fiber layer (RNFL), and ganglion cell complex (GCC) thickness and ONH parameters were obtained in vivo by OCT of the unaffected eye in dogs diagnosed with unilateral primary glaucoma (predisposed; n = 12) and compared with measures of healthy control eyes (normal; n = 13). Repeatability and intrarater reliability were explored using intraclass correlation coefficients (ICC).
Compared to normal eyes, predisposed eyes had a thinner retina in the temporal (P = 0.005), inferior quadrants (P = 0.003), and decreased inner retinal thickness (superior: P = 0.003, temporal: P = 0.001, inferior: P < 0.001, nasal: P = 0.001). Predisposed eyes had a thinner RNFL compared to normal eyes (P = 0.005), and when analyzed in quadrants, it was thinner in the superior (P < 0.001), temporal (P = 0.034), and nasal quadrants (P = 0.001). Repeatability (ICC 0.763-0.835) and intrarater reliability (ICC 0.824-0.942) were good to excellent for measures of retinal thickness and adequate for RNFL measurements (ICC 0.701-0.798). Reliable measurements of optic disk area were obtained and were similar between groups (P = 0.597). Measurements of parameters relying on automated software detection (GCC, optic cup, optic rim) had inadequate repeatability and reliability.
Statistically significant differences in retinal and RNFL thicknesses were identified in normal and predisposed eyes. Reliable and consistent measurements of variables with manual adjustment of software detected parameters were obtained. Validation of OCT as a diagnostic tool for clinical assessment in canine glaucoma is warranted.
使用光学相干断层扫描（OCT）评估易患青光眼的犬眼的视网膜和视神经乳头（ONH）。
25只眼（24只犬）。
通过OCT对诊断为单侧原发性青光眼的犬（易患组；n = 12）未受影响的眼睛进行活体测量，获取视乳头周围视网膜、视网膜神经纤维层（RNFL）和神经节细胞复合体（GCC）厚度以及ONH参数，并与健康对照眼（正常组；n = 13）的测量结果进行比较。使用组内相关系数（ICC）探讨重复性和评估者内可靠性。
与正常眼相比，易患眼在颞侧（P = 0.005）、下象限（P = 0.003）的视网膜较薄，视网膜内层厚度降低（上象限：P = 0.003，颞侧：P = 0.001，下象限：P < 0.001，鼻侧：P = 0.001）。与正常眼相比，易患眼的RNFL较薄（P = 0.005），按象限分析时，上象限（P < 0.001）、颞侧（P = 0.034）和鼻侧象限（P = 0.001）较薄。视网膜厚度测量的重复性（ICC 0.763 - 0.835）和评估者内可靠性（ICC 0.824 - 0.942）良好至优秀；RNFL测量的重复性和可靠性足够（ICC 0.701 - 0.798）。获得了可靠的视盘面积测量值，且两组间相似（P = 0.597）。依赖自动软件检测的参数（GCC、视杯、视盘边缘）测量的重复性和可靠性不足。
在正常眼和易患眼中，视网膜和RNFL厚度存在统计学显著差异。通过手动调整软件检测参数获得了可靠且一致的变量测量值。有必要验证OCT作为犬青光眼临床评估诊断工具的有效性。
