Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.
Eur Rev Med Pharmacol Sci. 2019 Jun;23(12):5206-5214. doi: 10.26355/eurrev_201906_18185.
Many studies have emphasized the function of microRNA-296 (miR-296) that inhibits tumor formation. To some extent, the role of miR-296 in esophageal squamous cell carcinoma (ESCC) remains misleading. Therefore, the current research was designed to investigate the regulatory mechanisms of miR-296 and signal transducer and activator of transcription 3 (STAT3) in ESCC.
The mRNA expression of miR-296-5p and STAT3 in ESCC tissues or cell lines was measured via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The protein level of STAT3 was measured by Western blotting assay. The Luciferase reporter assay was used to verify the binding sites between miR-296-5p and STAT3. The transwell assay was employed to identify cell migration and invasion.
Down-regulation of miR-296-5p was detected in ESCC tissues and cell lines (p<0.01). Additionally, miR-296-5p was found to target STAT3 directly. Functionally, up-regulation of miR-296-5p or down-regulation of STAT3 significantly inhibited cell migration and invasion in ESCC.
MiR-296-5p inhibited cell invasion and migration in ESCC by downregulating STAT3. The overexpression of miR-296-5p by targeting STAT3 suppressed tumorigenesis of ESCC cells.
许多研究强调了微小 RNA-296（miR-296）抑制肿瘤形成的功能。在某种程度上，miR-296 在食管鳞状细胞癌（ESCC）中的作用仍存在误导。因此，本研究旨在探讨 miR-296 与信号转导和转录激活因子 3（STAT3）在 ESCC 中的调控机制。
通过定量实时聚合酶链反应（qRT-PCR）测量 ESCC 组织或细胞系中 miR-296-5p 和 STAT3 的 mRNA 表达。通过 Western blot 测定 STAT3 的蛋白水平。荧光素酶报告基因检测用于验证 miR-296-5p 和 STAT3 之间的结合位点。Transwell 测定用于鉴定细胞迁移和侵袭。
在 ESCC 组织和细胞系中检测到 miR-296-5p 的下调（p<0.01）。此外，发现 miR-296-5p 可直接靶向 STAT3。功能上，上调 miR-296-5p 或下调 STAT3 可显著抑制 ESCC 中的细胞迁移和侵袭。
miR-296-5p 通过下调 STAT3 抑制 ESCC 中的细胞侵袭和迁移。通过靶向 STAT3 过表达 miR-296-5p 抑制 ESCC 细胞的肿瘤发生。
