miR-296-5p 通过下调 STAT3 信号通路抑制食管鳞癌细胞的侵袭和迁移。

MiR-296-5p inhibits cell invasion and migration of esophageal squamous cell carcinoma by downregulating STAT3 signaling.

机构信息

Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Jun;23(12):5206-5214. doi: 10.26355/eurrev_201906_18185.

DOI:10.26355/eurrev_201906_18185

PMID:31298412

Abstract

OBJECTIVE

Many studies have emphasized the function of microRNA-296 (miR-296) that inhibits tumor formation. To some extent, the role of miR-296 in esophageal squamous cell carcinoma (ESCC) remains misleading. Therefore, the current research was designed to investigate the regulatory mechanisms of miR-296 and signal transducer and activator of transcription 3 (STAT3) in ESCC.

PATIENTS AND METHODS

The mRNA expression of miR-296-5p and STAT3 in ESCC tissues or cell lines was measured via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The protein level of STAT3 was measured by Western blotting assay. The Luciferase reporter assay was used to verify the binding sites between miR-296-5p and STAT3. The transwell assay was employed to identify cell migration and invasion.

RESULTS

Down-regulation of miR-296-5p was detected in ESCC tissues and cell lines (p<0.01). Additionally, miR-296-5p was found to target STAT3 directly. Functionally, up-regulation of miR-296-5p or down-regulation of STAT3 significantly inhibited cell migration and invasion in ESCC.

CONCLUSIONS

MiR-296-5p inhibited cell invasion and migration in ESCC by downregulating STAT3. The overexpression of miR-296-5p by targeting STAT3 suppressed tumorigenesis of ESCC cells.

摘要

目的

许多研究强调了微小 RNA-296（miR-296）抑制肿瘤形成的功能。在某种程度上，miR-296 在食管鳞状细胞癌（ESCC）中的作用仍存在误导。因此，本研究旨在探讨 miR-296 与信号转导和转录激活因子 3（STAT3）在 ESCC 中的调控机制。

患者和方法

通过定量实时聚合酶链反应（qRT-PCR）测量 ESCC 组织或细胞系中 miR-296-5p 和 STAT3 的 mRNA 表达。通过 Western blot 测定 STAT3 的蛋白水平。荧光素酶报告基因检测用于验证 miR-296-5p 和 STAT3 之间的结合位点。Transwell 测定用于鉴定细胞迁移和侵袭。