Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China.
Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China; Department of Obstetrics, Xiamen Maternity and Child Health Care Hospital, Xiamen 361003, China.
Gene. 2019 Sep 10;713:143969. doi: 10.1016/j.gene.2019.143969. Epub 2019 Jul 9.
Ovarian cancer (OvCa) is one of the most lethal gynecologic malignancies worldwide. Pelvic and abdominal metastasis is a leading cause for the poor prognosis of OvCa patients. The relationship between long non-coding RNAs (lncRNAs) and OvCa remains unclear. Identifying key lncRNAs related with OvCa metastasis is crucial for research on the mechanism of OvCa metastasis. This study was designed to investigate the role of a novel lncRNA, which we named SOCAR, in serous OvCa.
LncRNA microarray and Real-time PCR were used to examine SOCAR expression in high grade serous ovarian cancer (HGSOC) and normal ovary tissues. The proliferation, migration and invasion of OvCa cell lines SKOV-3 and OVCAR-3 were analyzed by CCK-8, Transwell and Scratch wound healing assays. Western blotting was used to detect the expression of Wnt/β-catenin pathway-related proteins.
A novel serous OvCa-related lncRNA, SOCAR, was identified via microarray. SOCAR was overexpressed in primary HGSOC tumors compared with normal ovary tissues, and the expression of SOCAR correlated with progression in HGSOC patients. SOCAR also had higher expression in metastatic HGSOC tissues compared with primary cancer tissues. Moreover, upregulation of SOCAR promoted proliferation, migration and invasion in OvCa cells. Expression of Wnt1, β-catenin and MMP-9 were all increased by SOCAR overexpression.
SOCAR is related with HGSOC oncogenesis and progression. It may promote proliferation, migration and invasion in OvCa cells partially by upregulating MMP-9 through the Wnt/β-catenin pathway.
卵巢癌（OvCa）是全球最致命的妇科恶性肿瘤之一。盆腔和腹部转移是 OvCa 患者预后不良的主要原因。长链非编码 RNA（lncRNAs）与 OvCa 之间的关系尚不清楚。鉴定与 OvCa 转移相关的关键 lncRNA 对于研究 OvCa 转移的机制至关重要。本研究旨在探讨一种新型 lncRNA，我们将其命名为 SOCAR，在浆液性 OvCa 中的作用。
使用 lncRNA 微阵列和实时 PCR 检测高级别浆液性卵巢癌（HGSOC）和正常卵巢组织中 SOCAR 的表达。CCK-8、Transwell 和划痕愈合实验分析 OvCa 细胞系 SKOV-3 和 OVCAR-3 的增殖、迁移和侵袭能力。Western blot 检测 Wnt/β-catenin 通路相关蛋白的表达。
通过微阵列鉴定出一种新型浆液性 OvCa 相关 lncRNA，SOCAR。SOCAR 在原发性 HGSOC 肿瘤中的表达高于正常卵巢组织，并且 SOCAR 的表达与 HGSOC 患者的进展相关。SOCAR 在转移性 HGSOC 组织中的表达也高于原发性癌症组织。此外，SOCAR 的上调促进了 OvCa 细胞的增殖、迁移和侵袭。SOCAR 过表达后，Wnt1、β-catenin 和 MMP-9 的表达均增加。
SOCAR 与 HGSOC 的发生和进展有关。它可能通过上调 MMP-9 部分通过 Wnt/β-catenin 通路促进 OvCa 细胞的增殖、迁移和侵袭。
