Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, Shenyang, Liaoning, China (mainland).
Med Sci Monit. 2018 Mar 5;24:1329-1339. doi: 10.12659/msm.908456.
BACKGROUND Ovarian cancer is the most lethal malignant tumor of the female reproductive system, and the metastasis is one of the major factors that contribute to the poor outcome of patients with OC. Accumulating evidence indicates that lncRNAs are expressed and play important regulatory roles in ovarian cancer. MATERIAL AND METHODS Aberrant lncRNAs in primary ovarian cancer tissues (POCTs) and paired omental metastasis tissues (OMTs) of patients with HGSOC were studied via lncRNA microarray. Real-time PCR was performed to examine CTD-2020K17.1 expression in HGSOC tissues from 38 patients, a normal ovarian surface epithelium cell line, and 4 ovarian cancer cell lines. Additionally, Transwell assays, wound healing assays, CCK-8 proliferation assays, and flow cytometry were used to explore the biological function of CTD-2020K17.1 in ovarian cancer cells. Finally, Western blot analysis was used to verify the potential target gene of CTD-2020K17.1. RESULTS A novel lncRNA named CTD-2020K17.1 was identified via microarray analysis. Expression of CTD-2020K17.1 was significantly increased in OMTs and in 4 ovarian cancer cell lines compared with POCTs (P<0.05) or normal ovarian surface epithelial cell line (P<0.05). Moreover, CTD-2020K17.1 overexpression promoted migration, invasion, and proliferation of ovarian cancer cells, and CTD-2020K17.1 regulated the expression of CARD11. CONCLUSIONS CTD-2020K17.1 is significantly upregulated in OMTs and ovarian cancer cell lines. It can promote the migration, invasion, and proliferation of ovarian cancer cells, and CARD11 is regulated by CTD-2020K17.1.
卵巢癌是女性生殖系统中最致命的恶性肿瘤，转移是导致卵巢癌患者预后不良的主要因素之一。越来越多的证据表明，lncRNAs 在卵巢癌中表达并发挥重要的调节作用。
通过 lncRNA 微阵列研究了 HGSOC 患者的原发性卵巢癌组织（POCTs）和配对的大网膜转移组织（OMTs）中的异常 lncRNAs。对 38 名 HGSOC 患者、一个正常卵巢表面上皮细胞系和 4 个卵巢癌细胞系的 HGSOC 组织中的 CTD-2020K17.1 表达进行了实时 PCR 检测。此外，还进行了 Transwell 测定、划痕愈合测定、CCK-8 增殖测定和流式细胞术，以研究 CTD-2020K17.1 在卵巢癌细胞中的生物学功能。最后，通过 Western blot 分析验证了 CTD-2020K17.1 的潜在靶基因。
通过微阵列分析鉴定了一个新的 lncRNA ，命名为 CTD-2020K17.1。与 POCTs（P<0.05）或正常卵巢表面上皮细胞系（P<0.05）相比，CTD-2020K17.1 在 OMTs 和 4 个卵巢癌细胞系中的表达显著增加。此外，CTD-2020K17.1 的过表达促进了卵巢癌细胞的迁移、侵袭和增殖，并且 CTD-2020K17.1 调节了 CARD11 的表达。
CTD-2020K17.1 在 OMTs 和卵巢癌细胞系中显著上调。它可以促进卵巢癌细胞的迁移、侵袭和增殖，并且 CTD-2020K17.1 调节 CARD11 的表达。
