Department of Nephrology, Institute of Nephrology of Chongqing and Kidney Center of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
Department of Oncology, PLA 958 Hospital, Chongqing, 400020, China.
Mol Immunol. 2019 Aug;112:399-405. doi: 10.1016/j.molimm.2019.06.021. Epub 2019 Jul 9.
The spleen is an important secondary lymph organ. Splenomegaly induced by anemia could affect the function of spleen in immune responses. We observe that anemia induced in mice with reduced peripheral T cell trafficking to the spleen T cell zones as well as CCL21 and CCL19 expression. In accordance with previous research, we found that the production of EPO in the mice kidney was sharply increased post anemia. In addition, mice were injected with different doses of EPO. Our results show that with the increased dosage of EPO, the chemokine expression in the spleen is lowered with a decrease in peripheral T cell homing to the spleen T cell zones. At last, our results show that the anemia mice model administrated with anti-EPO antibody had a higher expression of spleen CCL19 and CCL21 and an increased count of periphery T cells trafficking to spleen T cell zones at day 3 post induction. These data indicate that anemia could disturb T cell movement in the spleen, which might further affect T cell immune response, with partial involvement of EPO.
脾脏是一个重要的次级淋巴器官。贫血引起的脾肿大可能会影响脾脏在免疫反应中的功能。我们观察到，在向脾脏 T 细胞区迁移的外周 T 细胞减少的贫血小鼠中，以及 CCL21 和 CCL19 的表达减少。与之前的研究一致，我们发现贫血后小鼠肾脏中 EPO 的产生急剧增加。此外，我们还向小鼠注射了不同剂量的 EPO。结果表明，随着 EPO 剂量的增加，脾脏趋化因子的表达降低，外周 T 细胞向脾脏 T 细胞区的归巢减少。最后，我们的结果表明，贫血小鼠模型给予抗 EPO 抗体后，脾脏 CCL19 和 CCL21 的表达更高，诱导后第 3 天外周 T 细胞向脾脏 T 细胞区的迁移增加。这些数据表明，贫血可能会扰乱脾脏中 T 细胞的运动，从而可能进一步影响 T 细胞免疫反应，其中部分涉及 EPO。
