Price Vision Group, Indianapolis, IN.
Clinica Baviera, Tarragona, Spain.
Cornea. 2019 Oct;38(10):1336-1338. doi: 10.1097/ICO.0000000000002051.
To describe a technique that ensures the production of a type 1 bubble when preparing pre-Descemet endothelial keratoplasty (PDEK) grafts with a high rate of predictability.
Donor corneas were placed on a support disc, and a blunt instrument was used to score 360 degrees of the peripheral Descemet membrane and endothelium just inside the trabecular meshwork. Air was injected in several short bursts and several stages with a 30-gauge needle on a 3-mL syringe 2.0 mm away from the limbus to create a type 1 big bubble. The technique was tested by 2 operators (M.S. and A.S.-J.) in 26 human donor corneas, including 12 for possible transplantation, over a 9-month period. Anterior segment optical coherence tomograph (AS-OCT) was performed in 1 case proving a type 1 bubble.
A type 1 big bubble was successfully created in 24 of 26 attempted cases (92.3%). The technique was used successfully to obtain PDEK tissue for transplant in 9 eyes. One case was not technically acceptable because of diffuse cell loss (>10%); however, the bubble preparation itself was successful. One case had a mixed bubble because of incomplete scoring, resulting in a Descemet membrane endothelial keratoplasty graft used for transplant. One case failed to form any bubble likely because the scoring was too central. Of a total of 26 cases, 14 cases were for practice.
The Soper technique significantly improved the success rate of creating a type 1 bubble for PDEK preparation.
描述一种技术，该技术可确保在制备预去内皮角膜内皮移植术（PDEK）移植物时产生 1 型气泡，且具有较高的可预测性。
将供体角膜置于支撑盘上，使用钝器械在小梁网内的周边角膜内皮层上划 360 度的线。用 30 号针头在距角膜缘 2.0mm 的 3ml 注射器上进行数次短时间、多阶段的空气注射，以形成 1 型大泡。该技术由 2 名操作人员（M.S.和 A.S.-J.）在 9 个月的时间里在 26 个人供体角膜中进行了测试，其中包括 12 个可能进行移植的角膜。在前节光学相干断层扫描（AS-OCT）中，1 例证实为 1 型气泡。
在 26 例尝试的病例中，有 24 例（92.3%）成功地制造出了 1 型大泡。该技术成功地用于 9 只眼获得 PDEK 组织进行移植。有 1 例因弥漫性细胞丢失（>10%）而在技术上不可接受；然而，气泡制备本身是成功的。有 1 例因不完全划线而出现混合泡，导致用于移植的角膜内皮层内皮移植术移植物。有 1 例未能形成任何气泡，可能是因为划线太中心了。在总共 26 例中，有 14 例是用于练习。
Soper 技术显著提高了制备 PDEK 时产生 1 型气泡的成功率。
