从马中分离的马链球菌兽疫亚种的遗传多样性。
Genetic diversity of Streptococcus equi subsp. zooepidemicus isolated from horses.
机构信息
School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, MC, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, Matelica, MC, Italy.
出版信息
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:7-13. doi: 10.1016/j.cimid.2019.03.012. Epub 2019 Mar 26.
Streptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic and zoonotic pathogen of horses. In this study, genetic intraspecies variability of SEZ obtained mainly from respiratory and genital samples of horses was investigated by analysis of the 16S-23S rRNA intergenic spacer region (ISR) and of the 16S rRNA gene. 16S-23S ISR rRNA type A1 was predominant, although a high rate of multiple products (30.5%) was obtained. Phylogenetic analysis of the 16S rRNA gene detected three genogroups (I, II and III). 16S rRNA variable regions V1 and V2 are the most important regions for evaluating SEZ intraspecies variability, but at least V1-V5 regions should be considered to avoid mistakes. Analysis of all 16S rRNA sequences available in databases assigned human SEZ to groups I and III but not to group II. These results show a high genetic variability in SEZ collected from different specimens of horses from various regions of Italy.
马链球菌兽亚种（SEZ）是一种机会性和人畜共患的马病原体。在这项研究中，通过分析 16S-23S rRNA 基因间隔区（ISR）和 16S rRNA 基因，研究了主要从马的呼吸道和生殖道样本中获得的 SEZ 的种内遗传变异性。A1 型 16S-23S ISR rRNA 占主导地位，尽管获得了高比例的多种产物（30.5%）。16S rRNA 基因的系统发育分析检测到三个基因群（I、II 和 III）。16S rRNA 可变区 V1 和 V2 是评估 SEZ 种内变异性的最重要区域，但至少应考虑 V1-V5 区域以避免错误。对数据库中所有可用的 16S rRNA 序列的分析将人类 SEZ 分配到 I 组和 III 组，但未分配到 II 组。这些结果表明，从意大利不同地区的不同马标本中采集的 SEZ 具有很高的遗传变异性。
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