甲型流感病毒 SLLTEVET 表位在丝状 M13 噬菌体上的多重展示及其免疫原性
Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage.
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
出版信息
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:76-80. doi: 10.1016/j.cimid.2019.05.004. Epub 2019 May 10.
Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens.
小肽需要大载体来刺激体液免疫系统。丝状噬菌体，如 M13，已被提议作为载体，在其衣壳表面表达和携带这些肽。流感病毒 A 型跨膜蛋白 M2 的 M2e 2-9（SLLTEVET）残基是保守的，被认为是针对广泛的流感病毒 A 株免疫的合适靶标。在这里，流感病毒 A 的 M2e（2-9）序列融合到 M13 噬菌体的主要外壳蛋白 gpVIII 的 N 端，并用于免疫肉鸡。结果表明，M13 噬菌体表面的 SLLTEVET 肽得到了表达，杂交噬菌体具有免疫原性，并在肉鸡中产生了针对 M2e（2-9）的特异性抗体。
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