State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
Toxins (Basel). 2022 Oct 4;14(10):683. doi: 10.3390/toxins14100683.
With the outbreak and spread of COVID-19, a deep investigation of SARS-CoV-2 is urgent. Direct usage of this virus for scientific research could provide reliable results and authenticity. However, it is strictly constrained and unrealistic due to its high pathogenicity and infectiousness. Considering its biosafety, different systems and technologies have been employed in immunology and biomedical studies. In this study, phage display technology was used to construct a nonpathogenic model for COVID-19 research. The nucleocapsid protein of SARS-CoV-2 was fused with the M13 phage capsid p3 protein and expressed on the M13 phages. After validation of its successful expression, its potential as the standard for qPCR quantification and affinity with antibodies were confirmed, which may show the possibility of using this nonpathogenic bacteriophage to replace the pathogenic virus in scientific research concerning SARS-CoV-2. In addition, the model was used to develop a system for the classification and identification of different samples using ATR-FTIR, which may provide an idea for the development and evaluation of virus monitoring equipment in the future.
随着 COVID-19 的爆发和传播，对 SARS-CoV-2 的深入研究迫在眉睫。直接使用该病毒进行科学研究可以提供可靠的结果和真实性。然而，由于其高致病性和传染性，这受到严格限制且不切实际。考虑到其生物安全性，在免疫学和生物医学研究中采用了不同的系统和技术。在这项研究中，噬菌体展示技术被用于构建 COVID-19 研究的无毒模型。SARS-CoV-2 的核衣壳蛋白与 M13 噬菌体衣壳 p3 蛋白融合并在 M13 噬菌体上表达。在验证其成功表达后，确认了其作为 qPCR 定量标准和与抗体亲和力的潜力，这可能表明使用这种无毒噬菌体替代 SARS-CoV-2 科学研究中的致病性病毒是可能的。此外，该模型还用于使用 ATR-FTIR 对不同样本进行分类和鉴定，这可能为未来病毒监测设备的开发和评估提供思路。
