IL-17A 产生的 CD8+CD103+皮肤归巢记忆 T 细胞在银屑病皮损中的意义及其与临床病程的可能关系。
Significance of IL-17A-producing CD8CD103 skin resident memory T cells in psoriasis lesion and their possible relationship to clinical course.
机构信息
Department of Dermatology, Hamamatsu University School of Medicine, Japan.
Department of Dermatology, Hamamatsu University School of Medicine, Japan.
出版信息
J Dermatol Sci. 2019 Jul;95(1):21-27. doi: 10.1016/j.jdermsci.2019.06.002. Epub 2019 Jun 20.
BACKGROUND
A number of studies have shown the relationship between the pathogenesis of psoriasis and skin resident memory T (T) cells.
OBJECTIVE
To investigate the cytokine profile of T cells from skin lesions of psoriasis and the relationship of skin T cells to the future clinical course of psoriasis.
METHODS
We used stocked samples of T cells that were ex vivo expanded from skin biopsies of 10 patients with psoriasis vulgaris. A half of 4-mm punch biopsy specimens was subjected to expansion of skin-infiltrating T cells using IL-2 and anti-CD3/CD28 antibody-coated microbeads. More than 10 T cells per specimen were stocked at -80°C. Defrosted cells were subjected to flow cytometric analysis. Another half of skin biopsies were subjected to immmunofluorescence staining for CD103 and other markers.
RESULTS
The biopsied skin revealed CD8CD103 T cells were present in the epidermis of psoriasis and associated with acanthosis. Sorted CD103 T cells were mostly CD8 memory T cells expressing CD69 with a skin-homing potential. A part of CD8CD103 T cells produced interferon-γ, IL-17A or IL-22. Notably, CD8CD103 T cells more frequently produced IL-17A than did CD8CD103 T cells. We retrospectively divided the 10 cases into the non-advanced therapy group, and the advanced therapy group in which systemic biologics or others were initiated within one year. The frequency of CD8CD103IL-17A T cells tended to be higher in the advanced therapy group.
CONCLUSION
These results suggest that IL-17A-producing CD8CD103 T cells are associated with a progressive clinical course of psoriasis.
背景
多项研究表明，银屑病的发病机制与皮肤固有记忆 T（T）细胞有关。
目的
探讨银屑病皮损 T 细胞的细胞因子谱及皮肤 T 细胞与银屑病未来临床病程的关系。
方法
我们使用了 10 例寻常型银屑病患者皮肤活检标本 ex vivo 扩增的 T 细胞储存样本。用 IL-2 和抗 CD3/CD28 抗体包被的微珠对 4-mm 皮肤活检标本的一半进行皮肤浸润 T 细胞的扩增。每例标本储存超过 10 个 T 细胞，在-80°C 保存。解冻的细胞进行流式细胞术分析。另一半皮肤活检标本进行 CD103 和其他标志物的免疫荧光染色。
结果
活检皮肤显示 CD8CD103 T 细胞存在于银屑病表皮中，并与棘层肥厚有关。分选的 CD103 T 细胞主要是表达 CD69 具有皮肤归巢潜能的 CD8 记忆 T 细胞。一部分 CD8CD103 T 细胞产生干扰素-γ、IL-17A 或 IL-22。值得注意的是，CD8CD103 T 细胞比 CD8CD103 T 细胞更频繁地产生 IL-17A。我们回顾性地将 10 例患者分为非先进治疗组和先进治疗组，即一年内开始全身生物制剂或其他治疗。先进治疗组中 CD8CD103IL-17A T 细胞的频率较高。
结论
这些结果表明，产生 IL-17A 的 CD8CD103 T 细胞与银屑病进行性临床病程有关。
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