Ohashi Y, Honda A, Iwai T, Mori Y
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Biochem Int. 1988 Feb;16(2):293-302.
Vanadate dose-dependently stimulated the incorporation of [3H]glucosamine into glycosaminoglycan, especially hyaluronic acid, in mesothelial cells from rabbit pericardium. The activity of hyaluronic acid synthase in the mesothelial cells treated with 50 microM vanadate for 0.5-1 h was stimulated to a level about 2 times over that of the control. Neither DNA synthesis nor protein synthesis in the mesothelial cells under the same experimental conditions was affected. The enhancement of the activity of hyaluronic acid synthase in the mesothelial cells treated with vanadate (50 microM) was not inhibited by the addition of cycloheximide (1 microgram/ml). These results suggest that vanadate stimulates the hyaluronic acid synthesis by activation of hyaluronic acid synthase in mesothelial cells from rabbit pericardium.
钒酸盐以剂量依赖的方式刺激[3H]葡糖胺掺入兔心包间皮细胞的糖胺聚糖中，尤其是透明质酸。用50微摩尔钒酸盐处理0.5 - 1小时的间皮细胞中，透明质酸合酶的活性被刺激到比对照高约2倍的水平。在相同实验条件下，间皮细胞中的DNA合成和蛋白质合成均未受到影响。添加环己酰亚胺（1微克/毫升）不会抑制用钒酸盐（50微摩尔）处理的间皮细胞中透明质酸合酶活性的增强。这些结果表明，钒酸盐通过激活兔心包间皮细胞中的透明质酸合酶来刺激透明质酸的合成。
