Olins D E, Olins A L, Sève A P, Bourgeois C A, Hubert J, Monsigny M
University of Tennessee Oak Ridge Graduate School of Biomedical Sciences 37830.
Biol Cell. 1988;62(1):95-8.
Upon incubation with fluoresceinylated neoglycoproteins, isolated macronuclei from the ciliated protozoan Euplotes eurystomus display different labelling patterns depending on the nature of the sugar bound to the neoglycoproteins. Specific sugar-binding components (i.e., lectin-like molecules) are associated with presumed nucleoli and with the macronuclear replication bands. This is the first demonstration that DNA synthesis and sugar-binding components are co-localized in an eukaryotic cell.
用荧光素标记的新糖蛋白孵育后,从纤毛虫类原生动物阔口游仆虫分离出的大核根据与新糖蛋白结合的糖的性质显示出不同的标记模式。特定的糖结合成分(即类凝集素分子)与假定的核仁以及大核复制带相关联。这是首次证明DNA合成和糖结合成分在真核细胞中共定位。