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1
Proliferating cell nuclear antigen/cyclin in the ciliate Euplotes eurystomus: localization in the replication band and in micronuclei.多核草履虫中增殖细胞核抗原/细胞周期蛋白：在复制带和微核中的定位
J Cell Biol. 1989 Oct;109(4 Pt 1):1399-410. doi: 10.1083/jcb.109.4.1399.
2
Differential distribution of alpha-tubulin isotypes in Euplotes eurystomus determined by confocal immunofluorescence microscopy.通过共聚焦免疫荧光显微镜确定的宽口游仆虫中α-微管蛋白亚型的差异分布。
Biol Cell. 1989;66(3):235-46.
3
In vitro DNA synthesis in the macronuclear replication band of Euplotes eurystomus.真核阔口游仆虫大核复制带中的体外DNA合成
J Cell Biol. 1987 May;104(5):1125-32. doi: 10.1083/jcb.104.5.1125.
4
Stereo-electron microscopy of nuclear structure and replication in ciliated protozoa (Hypotricha).纤毛虫（腹毛目）细胞核结构与复制的立体电子显微镜观察
Eur J Cell Biol. 1981 Aug;25(1):120-30.
5
Lectin-like components in the macronuclear replication bands of Euplotes eurystomus.阔口游仆虫大核复制带中的类凝集素成分。
Biol Cell. 1988;62(1):95-8.
6
Replication bands and nucleoli in the macronucleus of Euplotes eurystomus: an ultrastructural and cytochemical study.真宽扁裸藻大核中的复制带和核仁：一项超微结构和细胞化学研究
Biol Cell. 1988;62(1):83-93.
7
Nucleus-specific and temporally restricted localization of proteins in Tetrahymena macronuclei and micronuclei.蛋白质在四膜虫大核和小核中的细胞核特异性及时间限制定位。
J Cell Biol. 1989 Nov;109(5):1983-92. doi: 10.1083/jcb.109.5.1983.
8
Exploiting nuclear duality of ciliates to analyse topological requirements for DNA replication and transcription.利用纤毛虫的核二元性分析DNA复制和转录的拓扑学要求。
J Cell Sci. 2005 Sep 1;118(Pt 17):3973-83. doi: 10.1242/jcs.02497.
9
Gene for proliferating-cell nuclear antigen (DNA polymerase delta auxiliary protein) is present in both mammalian and higher plant genomes.增殖细胞核抗原（DNA聚合酶δ辅助蛋白）基因存在于哺乳动物和高等植物基因组中。
Proc Natl Acad Sci U S A. 1989 May;86(9):3189-93. doi: 10.1073/pnas.86.9.3189.
10
Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry.针对增殖细胞核抗原（PCNA）/细胞周期蛋白的单克隆抗体，作为通过免疫荧光显微镜和流式细胞术检测增殖细胞的探针。
J Immunol Methods. 1988 Apr 22;109(1):49-59. doi: 10.1016/0022-1759(88)90441-3.

引用本文的文献

1
Regulating telomere length from the inside out: the replication fork model.从内而外调节端粒长度：复制叉模型。
Genes Dev. 2016 Jul 1;30(13):1483-91. doi: 10.1101/gad.280578.116.
2
Relative paradigms between autoantibodies in lupus and autoantibodies in cancer.狼疮自身抗体与癌症自身抗体之间的相关范例。
Clin Exp Immunol. 2003 Nov;134(2):169-77. doi: 10.1046/j.1365-2249.2003.02259.x.
3
rTP: a candidate telomere protein that is associated with DNA replication.rTP：一种与DNA复制相关的候选端粒蛋白。
Chromosoma. 1996 Aug;105(2):82-91. doi: 10.1007/BF02509517.
4
The DNA of ciliated protozoa.纤毛虫的DNA。
Microbiol Rev. 1994 Jun;58(2):233-67. doi: 10.1128/mr.58.2.233-267.1994.
5
Telomerase RNA localized in the replication band and spherical subnuclear organelles in hypotrichous ciliates.端粒酶RNA定位于下毛目纤毛虫的复制带和球形亚核细胞器中。
J Cell Biol. 1995 Jul;130(2):243-53. doi: 10.1083/jcb.130.2.243.
6
Inaccessibility of the Euplotes telomere binding protein.真核草履虫端粒结合蛋白难以获取。
Chromosoma. 1993 Dec;102(10):700-11. doi: 10.1007/BF00650896.
7
Structure of the macronuclear polyubiquitin gene in Euplotes.真核草履虫大核多聚泛素基因的结构
Chromosoma. 1991 Jul;100(6):386-94. doi: 10.1007/BF00337517.

本文引用的文献

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Comparison between the timing of micronuclear and macronuclear DNA synthesis in Euplotes eurystomus.真宽扁虫微核与大核DNA合成时间的比较。
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2
Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation.一种细胞核和细胞质多肽的鉴定，其相对比例对细胞增殖速率的变化敏感。
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Structural interaction of cytoskeletal components.细胞骨架成分的结构相互作用。
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A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.对整个海拉细胞周期中差异多肽合成的研究。
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Stereo-electron microscopy of nuclear structure and replication in ciliated protozoa (Hypotricha).纤毛虫（腹毛目）细胞核结构与复制的立体电子显微镜观察
Eur J Cell Biol. 1981 Aug;25(1):120-30.
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Identity of the proliferating cell nuclear antigen and cyclin.增殖细胞核抗原与细胞周期蛋白的同一性。
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Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.狼疮血清中自身抗体识别的增殖细胞核抗原的特征分析。
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8
Cytochemistry of the chromatin replication band in hypotrichous ciliated protozoa staining with silver and thiol-specific coumarin maleimide.用银和硫醇特异性香豆素马来酰亚胺染色的腹毛目纤毛虫中染色质复制带的细胞化学
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Disruption of DNA synthesis in Euplotes by heat shock.热休克对真核草履虫DNA合成的干扰。
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10
Electron microscope autoradiography of DNA synthesis in the replication band of two hypotrichous ciliates.两种下毛目纤毛虫复制带中DNA合成的电子显微镜放射自显影
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多核草履虫中增殖细胞核抗原/细胞周期蛋白：在复制带和微核中的定位

Proliferating cell nuclear antigen/cyclin in the ciliate Euplotes eurystomus: localization in the replication band and in micronuclei.

作者信息

Olins D E, Olins A L, Cacheiro L H, Tan E M

机构信息

University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences 37831.

出版信息

J Cell Biol. 1989 Oct;109(4 Pt 1):1399-410. doi: 10.1083/jcb.109.4.1399.

DOI:10.1083/jcb.109.4.1399
PMID:2477376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2115802/
Abstract

Human autoimmune sera specific for proliferating cell nuclear antigen (PCNA)/cyclin (auxiliary protein for DNA polymerase delta) demonstrated the presence of epitopes within the macro- and micronuclei of the hypotrichous ciliated protozoa Euplotes eurystomus. Tightly bound PCNA/cyclin was localized at the site of DNA synthesis in macronuclei, the rear zone of the replication band. Starvation or heat shock, conditions that reduce macronuclear replication, resulted in a decrease of PCNA/cyclin in replication bands. Micronuclei also exhibited PCNA/cyclin localization which persisted for a large proportion of the vegetative cell cycle and exhibited significant resistance to adverse culture conditions. Immunoprecipitation of 35S-labeled soluble Euplotes proteins with PCNA/cyclin autoimmune sera revealed a spectrum of low molecular mass proteins. PCNA/cyclin-like proteins have now been observed in the widely divergent species: human, rat, amphibian, yeast, and ciliated protozoa.

摘要

针对增殖细胞核抗原（PCNA）/细胞周期蛋白（DNA聚合酶δ的辅助蛋白）的人自身免疫血清显示，在多核纤毛虫阔口游仆虫的大核和小核内存在表位。紧密结合的PCNA/细胞周期蛋白定位于大核中DNA合成的位点，即复制带的后部区域。饥饿或热休克这些会减少大核复制的条件，导致复制带中PCNA/细胞周期蛋白减少。小核也表现出PCNA/细胞周期蛋白定位，这种定位在大部分营养细胞周期中持续存在，并且对不利的培养条件表现出显著抗性。用PCNA/细胞周期蛋白自身免疫血清对35S标记的游仆虫可溶性蛋白进行免疫沉淀，揭示了一系列低分子量蛋白。现已在广泛不同的物种中观察到PCNA/细胞周期蛋白样蛋白：人类、大鼠、两栖动物、酵母和纤毛虫。