Department of Animal Science, Aarhus University Foulum, 8830, Tjele, Denmark.
Department of Animal Science, Aarhus University Foulum, 8830, Tjele, Denmark.
Reprod Toxicol. 2019 Oct;89:115-123. doi: 10.1016/j.reprotox.2019.07.006. Epub 2019 Jul 10.
Litter size and other conventional measures in rodents are common end-points in the assessment of xenobiotics for reprotoxic effects. However, since litter size may be normal despite reduced semen quality, we established and tested a mouse in vitro fertilization/in vitro culture (IVF/IVC) system to assess other aspects of reprotoxicity of xenobiotic exposure. Two pesticides, vinclozolin (V) and chlormequat (C), were added to feed in low (40 and 900 ppm, respectively) and high (300 and 2700 ppm, respectively) doses and compared to control (nil pesticide). Exposed males were used for natural mating to evaluate litter size and then used for IVF/IVC and sperm evaluation. The IVF/IVC system detected significant adverse effect of high dose of vinclozolin on blastocyst formation, which was not detected by conventional measures such as litter size or sperm motility and viability. We conclude that assessment based on IVF/IVC measures may complement litter size and other conventional end-points.
胎仔数和其他传统的啮齿类动物指标常用于评估外源化学物的生殖毒性效应。然而，由于精液质量降低但胎仔数可能正常，我们建立并测试了一种用于评估外源化学物暴露生殖毒性其他方面的体外受精/体外培养（IVF/IVC）系统。两种农药，乙烯菌核利（V）和矮壮素（C），分别以低剂量（分别为 40 和 900ppm）和高剂量（分别为 300 和 2700ppm）添加到饲料中，并与对照组（无农药）进行比较。暴露的雄性用于自然交配以评估胎仔数，然后用于 IVF/IVC 和精子评估。IVF/IVC 系统检测到高剂量乙烯菌核利对囊胚形成的显著不良影响，而传统指标如胎仔数或精子运动能力和活力则无法检测到。我们得出结论，基于 IVF/IVC 措施的评估可能补充胎仔数和其他传统终点。
