Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Bioresour Technol. 2019 Oct;290:121725. doi: 10.1016/j.biortech.2019.121725. Epub 2019 Jun 29.
A salt-tolerant aerobic denitrifying bacterium, Zobellella denitrificans strain A63, was isolated, and its effects on the efficiency of denitrification of saline wastewater and the denitrifying microbial community structure in the matrix were studied in vertical-flow constructed wetlands (VFCWs). In a VFCW system with strain A63, the removal efficiencies of NH-N, NO-N, and total nitrogen reached 79.2%, 95.7%, and 89.9%, respectively. Quantitative PCR analysis indicated that the amoA gene from ammonia-oxidizing archaea (AOA) was highly abundant, whereas amoA from ammonia-oxidizing bacteria and nxrA from nitrite-oxidizing bacteria were lowly abundant because of the influent salinity, irrespective of whether strain A63 was added. However, the addition of strain A63 significantly increased the abundance of nirK in the top layer of the VFCW. Therefore, AOA-driven partial nitrification and aerobic denitrification by strain A63 occurred in VFCWs. Our findings suggest that adding salt-tolerant denitrifying strains to constructed wetlands can enhance denitrification for saline wastewater treatment.
一株耐盐好氧反硝化菌 Zobellella denitrificans 菌株 A63 被分离出来，并在垂直流人工湿地（VFCWs）中研究了其对含盐废水脱氮效率和基质中脱氮微生物群落结构的影响。在含有菌株 A63 的 VFCW 系统中，NH-N、NO-N 和总氮的去除效率分别达到 79.2%、95.7%和 89.9%。定量 PCR 分析表明，由于进水盐度的影响，氨氧化古菌（AOA）的 amoA 基因高度丰富，而氨氧化细菌的 amoA 和亚硝酸盐氧化细菌的 nxrA 则很少，而不论是否添加菌株 A63。然而，添加菌株 A63 显著增加了 VFCW 顶层 nirK 的丰度。因此，AOA 驱动的部分硝化和菌株 A63 进行的好氧反硝化作用发生在 VFCWs 中。我们的研究结果表明，向人工湿地中添加耐盐脱氮菌株可以增强对含盐废水的脱氮处理。
