Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Bioresour Technol. 2021 Sep;335:125236. doi: 10.1016/j.biortech.2021.125236. Epub 2021 Apr 30.
Six laboratory-scale constructed wetlands (CWs) were used to quantify the nitrogen removal (NR) capacity in the treatment of saline wastewater at high (6:1) and low (2:1) carbon-nitrogen ratios (C/N), with and without bioaugmentation of aerobic-denitrifying bacterium. Sustained high-efficiency nitrification was observed throughout the operation. However, under different C/N ratios, although the bioaugmentation of aerobic-denitrifying bacterium promoted the removal of NO-N and TN, there were still great differences in denitrification. Molecular biology experiments revealed ammonia-oxidizing archaea, together with the Nitrosomonas and Nitrospira, led to highly efficient nitrification. Furthermore, aerobic-denitrifying bacterium and sulfur-driven denitrifiers were the core denitrification groups in CWs. By performing these combined experiments, it was possible to determine the optimal CW design and the most relevant NR processes for the treatment of salty wastewater. The results suggest that the bioaugmentation of salt-tolerant functional bacteria with multiple NR pathways are crucial for the removal of salty wastewater pollutants.
六个实验室规模的人工湿地 (CW) 被用于量化在高 (6:1) 和低 (2:1) 碳氮比 (C/N) 条件下，以及有氧反硝化细菌生物强化时，处理含盐废水的氮去除 (NR) 能力。在整个运行过程中观察到持续的高效硝化作用。然而，在不同的 C/N 比下，尽管有氧反硝化细菌的生物强化促进了 NO-N 和 TN 的去除，但仍存在很大的脱氮差异。分子生物学实验表明，氨氧化古菌与亚硝化单胞菌和硝化螺旋菌一起导致了高效的硝化作用。此外，有氧反硝化细菌和硫驱动的反硝化菌是 CW 中的核心脱氮菌。通过进行这些组合实验，可以确定处理含盐废水的最佳 CW 设计和最相关的 NR 工艺。结果表明，具有多种 NR 途径的耐盐功能细菌的生物强化对于去除含盐废水中的污染物至关重要。
