Brower D L
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Development. 1987 Sep;101(1):83-92. doi: 10.1242/dev.101.1.83.
Using a monoclonal antibody and image-processing procedures, the patterns of expression of the Ultrabithorax (Ubx) gene product have been characterized in Drosophila larvae. As reported previously, the metathoracic imaginal discs stain most intensely with anti-Ubx, with some mesothoracic and no prothoracic expression detectable. In the metathoracic discs, the greatest modulation in anti-Ubx staining is along the proximodistal axis. Ubx is generally expressed at higher levels in the posterior regions of metathoracic discs, although relatively high anterior expression is found in some areas. Expression in the mature wing disc is confined to the squamous peripodial membrane cells; in younger wings, Ubx expression fills the posterior half of the peripodial side of the disc. The mesothoracic leg stains with a pattern that is qualitatively similar (but not identical) to that of the metathoracic leg; Ubx is expressed in some anterior regions of the mesothoracic leg, in parasegment 4. Double staining with anti-Ubx and anti-engrailed reveals that discontinuities in Ubx expression that have been suggested to correspond to compartment borders do not coincide with the compartment boundaries in some cases. In the larval ventral ganglion, Ubx expression is greatest in parasegments 5 and 6, as in the embryonic nervous system.
利用单克隆抗体和图像处理程序，已对果蝇幼虫中超双胸节（Ubx）基因产物的表达模式进行了表征。如先前报道，后胸成虫盘用抗Ubx抗体染色最深，中胸有一些表达，而前胸未检测到表达。在后胸盘中，抗Ubx染色的最大调节沿近端-远端轴。Ubx通常在后胸盘的后部区域表达水平较高，尽管在某些区域发现前部表达相对较高。在成熟翅盘中的表达局限于鳞状围膜细胞；在较年轻的翅中，Ubx表达充满盘围膜侧的后半部分。中胸腿的染色模式与后胸腿在质量上相似（但不完全相同）；Ubx在中胸腿的一些前部区域、副节4中表达。用抗Ubx和抗engrailed双染色显示，曾被认为与分隔边界相对应的Ubx表达间断在某些情况下与分隔边界不一致。在幼虫腹神经节中，Ubx表达在副节5和6中最高，与胚胎神经系统中的情况一样。
