Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
J Ind Microbiol Biotechnol. 2019 Nov;46(11):1569-1581. doi: 10.1007/s10295-019-02219-w. Epub 2019 Jul 13.
To investigate the differences in microbial community structure between mesophilic and thermophilic in situ biogas-upgrading systems by H addition, two reactors (35 °C and 55 °C) were run for four stages according to different H addition rates (H/CO of 0:1, 1:1, and 4:1) and mixing mode (intermittent and continuous). 16S rRNA gene-sequencing technology was applied to analyze microbial community structure. The results showed that the temperature is a crucial factor in impacting succession of microbial community structure and the H utilization pathway. For mesophilic digestion, most of added H was consumed indirectly by the combination of homoacetogens and strict aceticlastic methanogens. In the thermophilic system, most of added H may be used for microbial cell growth, and part of H was utilized directly by strict hydrogenotrophic methanogens and facultative aceticlastic methanogens. Continuous stirring was harmful to the stabilization of mesophilic system, but not to the thermophilic one.
为了通过 H 供体来研究中温和高温原位沼气升级系统中微生物群落结构的差异，根据不同的 H/CO 比（0:1、1:1 和 4:1）和混合方式（间歇和连续），两个反应器（35°C 和 55°C）经过四个阶段运行。应用 16S rRNA 基因测序技术分析微生物群落结构。结果表明，温度是影响微生物群落结构和 H 利用途径演替的关键因素。对于中温消化，大部分添加的 H 通过同型产乙酸菌和严格乙酸分解产甲烷菌的结合被间接消耗。在高温系统中，大部分添加的 H 可能用于微生物细胞生长，部分 H 被严格氢营养型产甲烷菌和兼性乙酸分解产甲烷菌直接利用。连续搅拌对中温系统的稳定不利，但对高温系统没有影响。
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