INRAE, University of Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, France.
French Environment and Energy Management Agency, 20 Avenue du Grésillé- BP 90406, CEDEX 01, 49004 Angers, France.
Molecules. 2020 Dec 1;25(23):5665. doi: 10.3390/molecules25235665.
The conversion of H into methane can be carried out by microorganisms in a process so-called biomethanation. In ex-situ biomethanation H and CO gas are exogenous to the system. One of the main limitations of the biomethanation process is the low gas-liquid transfer rate and solubility of H which are strongly influenced by the temperature. Hydrogenotrophic methanogens that are responsible for the biomethanation reaction are also very sensitive to temperature variations. The aim of this work was to evaluate the impact of temperature on batch biomethanation process in mixed culture. The performances of mesophilic and thermophilic inocula were assessed at 4 temperatures (24, 35, 55 and 65 °C). A negative impact of the low temperature (24 °C) was observed on microbial kinetics. Although methane production rate was higher at 55 and 65 °C (respectively 290 ± 55 and 309 ± 109 mL CH/L.day for the mesophilic inoculum) than at 24 and 35 °C (respectively 156 ± 41 and 253 ± 51 mL CH/L.day), the instability of the system substantially increased, likely because of a strong dominance of only species. Considering the maximal methane production rates and their stability all along the experiments, an optimal temperature range of 35 °C or 55 °C is recommended to operate ex-situ biomethanation process.
H 到甲烷的转化可以通过微生物在所谓的生物甲烷化过程中完成。在异位生物甲烷化中，H 和 CO 气体是系统外源性的。生物甲烷化过程的主要限制之一是 H 的气液传质速率和溶解度低，这强烈受到温度的影响。负责生物甲烷化反应的氢营养型产甲烷菌对温度变化也非常敏感。这项工作的目的是评估温度对混合培养物分批生物甲烷化过程的影响。在 4 个温度（24、35、55 和 65°C）下评估了中温和嗜热接种物的性能。低温（24°C）对微生物动力学产生了负面影响。尽管在 55 和 65°C 时甲烷生成率更高（分别为中温接种物的 290±55 和 309±109 mL CH/L.day），而在 24 和 35°C 时（分别为 156±41 和 253±51 mL CH/L.day），但系统的不稳定性大大增加，这可能是因为只有 种的强烈优势。考虑到最大甲烷生成速率及其在整个实验过程中的稳定性，建议在 35°C 或 55°C 的最佳温度范围内运行异位生物甲烷化过程。
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