Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117350. doi: 10.1016/j.saa.2019.117350. Epub 2019 Jul 8.
The intracellular pH has a significant effect on several essential biological processes such as material transfer, enzymatic action, cell apoptosis et al. Thus, it is necessary to monitor pH fluctuation in living cells. Here, we designed a near-infrared ratiometric fluorescence probe for pH detection. The spectroscopic responses of probe to pH variations were investigated in CHOH/PBS (v/v, 1:1) mixed solution at different pH values. The experimental results showed that the probe is sensitive to acidity, and the pK of probe is calculated to be 4.85. When the pH was decreased from 9.0 to 1.0, the color of probe solution change from purple to yellow was found by naked eye. Moreover, the probe can be a practical tool for assessing cellular pH by cell imaging.
细胞内 pH 值对许多重要的生物过程有显著影响，如物质传递、酶促反应、细胞凋亡等。因此，有必要监测活细胞内的 pH 值波动。在这里，我们设计了一种近红外比率荧光探针用于 pH 值检测。在不同 pH 值下，在 CHOH/PBS（v/v，1:1）混合溶液中研究了探针对 pH 值变化的光谱响应。实验结果表明，该探针对酸度敏感，探针的 pK 值计算为 4.85。当 pH 值从 9.0 降低到 1.0 时，探针溶液的颜色通过肉眼观察从紫色变为黄色。此外，该探针可通过细胞成像成为评估细胞 pH 值的实用工具。
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