Key Laboratory of Science and Technology of Eco-Textiles, Ministry of Education, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai, 201620, China.
College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, China.
J Fluoresc. 2021 Jul;31(4):1133-1141. doi: 10.1007/s10895-021-02741-0. Epub 2021 May 11.
A new spiropyran-based fluorescent probe was developed for dual detection of Fe ion and pH. Addition of Fe and Ag to the probe solution enhanced the fluorescence intensity by 6 and 5 fold, respectively. Addition of Fe, Hg and Ni caused slight increase in the fluorescence intensity of the probe. While addition of other common metal ions did not bring about substantial change of the fluorescence. Thus the probe can be used for fluorescence turn-on detection of Fe ion in ethanol/water (9:1) medium. The detection limit of the probe for Fe is 0.77 µM. The suitable pH range for the probe to detect Fe was pH 3 - 9. Other metal ions including Li, Na, K, Ag, Cu, Zn, Co, Ni, Mn, Sr, Hg, Ca, Mg, Al, Cr, and Fe did not cause marked interference with Fe recognition. The color of the probe solution was yellow at pH 1 - 2 and colorless at other pH values. The fluorescence intensity of the probe was low at pH 1 - 12 and increased significantly when the pH was 13 and 14, indicating that the probe can be used as a colorimetric and fluorescent probe for sensing extremely acidic or extremely alkaline conditions through different channels.
一种基于新型螺吡喃的荧光探针被开发用于同时检测 Fe 离子和 pH。向探针溶液中加入 Fe 和 Ag ，分别使荧光强度增强了 6 倍和 5 倍。加入 Fe、Hg 和 Ni 会使探针的荧光强度略有增加。而加入其他常见金属离子则不会使荧光发生实质性变化。因此，该探针可用于乙醇/水（9:1）介质中 Fe 离子的荧光开启检测。探针对 Fe 的检测限为 0.77µM。该探针检测 Fe 的适宜 pH 范围为 pH 3-9。其他金属离子，包括 Li、Na、K、Ag、Cu、Zn、Co、Ni、Mn、Sr、Hg、Ca、Mg、Al、Cr 和 Fe，对 Fe 的识别没有明显干扰。探针溶液在 pH 1-2 时为黄色，在其他 pH 值时为无色。探针在 pH 1-12 时的荧光强度较低，当 pH 为 13 和 14 时，荧光强度显著增加，表明该探针可通过不同通道用作极酸或极碱条件的比色和荧光探针。
