Poolman J T, Timmermans H A, Hopman C T, Teerlink T, Van Vught P A, Witvliet M H, Beuvery E C
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Antonie Van Leeuwenhoek. 1987;53(6):413-9. doi: 10.1007/BF00415495.
Outer membrane proteins (OMPs) were isolated from meningococcal strain H44/76 (B:15:P1.16) by detergent extraction of bacteria. A final product containing class 1 (P1.16), 3(15), 4 OMPs and 5% (w/w) lipooligosaccharide was obtained. Two experimental vaccines were prepared: OMP-detergent and OMP-C polysaccharide. The OMP-detergent vaccine tended to show a better bactericidal: ELISA ratio for the antibodies induced as compared to the OMP-C polysaccharide vaccine. The vaccine induced bactericidal antibodies appeared for the greater part to be directed against the class 1 OMP (P1.16). By comparison of cultures grown in Mueller Hinton Broth with and without 0.25% (w/v) glucose, it was found that monoclonal antibodies against the serotype OMP (class 2 or 3) were not bactericidal against meningococci grown in MHB without glucose. Antibodies against class 1 OMP and lipooligosaccharide were not influenced by this. A new major outer membrane protein (appr. 40 kd) is described that may function as a cation-specific porin.
通过去污剂提取细菌，从脑膜炎球菌菌株H44/76（B:15:P1.16）中分离出外膜蛋白（OMPs）。获得了一种最终产物，其中包含1类（P1.16）、3类（15）、4类OMPs和5%（w/w）脂寡糖。制备了两种实验性疫苗：OMP-去污剂疫苗和OMP-C多糖疫苗。与OMP-C多糖疫苗相比，OMP-去污剂疫苗诱导产生的抗体的杀菌：ELISA比值往往更高。疫苗诱导产生的杀菌抗体大部分似乎是针对1类OMP（P1.16）的。通过比较在添加和不添加0.25%（w/v）葡萄糖的穆勒-欣顿肉汤中培养的细菌，发现针对血清型OMP（2类或3类）的单克隆抗体对在不含葡萄糖的MHB中生长的脑膜炎球菌没有杀菌作用。针对1类OMP和脂寡糖的抗体不受此影响。描述了一种新的主要外膜蛋白（约40kd），它可能作为一种阳离子特异性孔蛋白发挥作用。
