Morse S A, Mintz C S, Sarafian S K, Bartenstein L, Bertram M, Apicella M A
Infect Immun. 1983 Jul;41(1):74-82. doi: 10.1128/iai.41.1.74-82.1983.
Growth of Neisseria gonorrhoeae strain FA171 in continuous culture under glucose-limiting conditions resulted in a growth-rate-dependent change in the lipopolysaccharide (LPS). The evidence for this change is an alteration in the mobility of purified alkali-treated LPS on sodium dodecyl sulfate-polyacrylamide gels and a quantitative difference in the amount of the LPS serotype antigen. The LPS from cells grown at a low dilution rate (0.12 h-1) contained ca. eightfold less serotype antigen than the LPS from cells grown at a high dilution rate (0.56 h-1). The decrease in LPS serotype antigen was associated with an increase in sensitivity to the bactericidal activity of normal human serum and an increase in cell surface hydrophobicity. An increase in the amount of serotype antigen was associated with a reduction in the accessibility of a monoclonal antibody to a core LPS determinant, an increase in resistance to normal human serum, and a decrease in cell surface hydrophobicity. The microheterogeneity of gonococcal LPS with respect to the content of serotype antigen may result from an alteration in the metabolism of glucose.
淋病奈瑟菌菌株FA171在葡萄糖限制条件下进行连续培养时，其脂多糖（LPS）会出现与生长速率相关的变化。这种变化的证据包括：纯化的经碱处理的LPS在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上的迁移率改变，以及LPS血清型抗原量的定量差异。以低稀释率（0.12 h⁻¹）生长的细胞产生的LPS所含血清型抗原比以高稀释率（0.56 h⁻¹）生长的细胞产生的LPS少约八倍。LPS血清型抗原的减少与对正常人血清杀菌活性的敏感性增加以及细胞表面疏水性增加有关。血清型抗原量的增加与单克隆抗体对核心LPS决定簇的可及性降低、对正常人血清的抗性增加以及细胞表面疏水性降低有关。淋球菌LPS在血清型抗原含量方面的微观异质性可能是由葡萄糖代谢的改变引起的。
