Umemura Y, Huskey R A, Anderson J M
Department of Macromolecular Science, Case Western Reserve University, Cleveland, OH 44106.
Biomaterials. 1988 Mar;9(2):133-7. doi: 10.1016/0142-9612(88)90111-1.
In vitro human platelet interactions with surfaces of type I collagen, chondroitin-6-sulphate (CH-6-S), chondroitin-4-sulphate (CH-4-S), a CH-6-S/collagen layer, and a collagen-CH-6-S complex were investigated. Polystyrene and silanized glass served as controls. Platelet counts, platelet factor 4 released, and platelet aggregating ability for the different surfaces were compared with controls. Platelet count and platelet factor 4 release data showed that there were no differences between surfaces of CH-6-S, CH-4-S and the controls. However, significant differences in platelet counts and platelet factor 4 released were found when collagen, the CH-6-S/collagen layer, and the collagen-CH-6-S complex were compared with controls. The pure type I collagen surface had the greatest influence on platelet activation. The collagen-CH-6-S complex had a greater effect that the CH-6-S/collagen layer on platelet activation. It appears that chondroitin-6-sulphate can modify the platelet activity of type I collagen.
研究了体外人血小板与I型胶原蛋白、硫酸软骨素-6-硫酸盐（CH-6-S）、硫酸软骨素-4-硫酸盐（CH-4-S）、CH-6-S/胶原蛋白层以及胶原蛋白-CH-6-S复合物表面的相互作用。聚苯乙烯和硅烷化玻璃作为对照。将不同表面的血小板计数、释放的血小板因子4以及血小板聚集能力与对照进行比较。血小板计数和血小板因子4释放数据表明，CH-6-S、CH-4-S表面与对照之间没有差异。然而，当将胶原蛋白、CH-6-S/胶原蛋白层以及胶原蛋白-CH-6-S复合物与对照进行比较时，发现血小板计数和释放的血小板因子4存在显著差异。纯I型胶原蛋白表面对血小板活化的影响最大。胶原蛋白-CH-6-S复合物对血小板活化的作用比CH-6-S/胶原蛋白层更大。看来硫酸软骨素-6-硫酸盐可以改变I型胶原蛋白的血小板活性。
