Godard A, Gascan H, Naulet J, Peyrat M A, Jacques Y, Soulillou J P, Moreau J F
National de la Santé et de la Recherche Médicale (U 211-Unité de Recherche sur les Effecteurs Lymphocytaires T), Faculté de Médecine, Nantes, France.
Blood. 1988 Jun;71(6):1618-23.
We previously described a lymphokine termed HILDA (for human interleukin DA) produced by T-lymphocyte alloreactive clones after antigenic stimulation. This factor sustains the growth of a murine IL3-sensitive cell line (DA2). In addition, HILDA is a potent activator of eosinophils and displays a burst-promoting activity on human bone marrow. In the present study, HILDA was purified to homogeneity from T-cell clone supernatant using successively sequential concentration, concanavalin A (ConA) affinity chromatography with differential elution (alpha-D glucopyranoside and alpha-D mannopyranoside), high-performance liquid chromatography (HPLC) gel filtration and reverse-phase HPLC. The pure material appeared as a 38-kd glycoprotein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing or nonreducing conditions. Biologic activity could be recovered from SDS-PAGE gel slices corresponding to the 38-kd band. We conclude from the specificity of the DA-2 cell line and biochemical characteristics described that this lymphokine is different from other known factors produced by human T lymphocytes.
我们之前描述了一种名为HILDA（人类白细胞介素DA）的淋巴因子，它由T淋巴细胞同种异体反应性克隆在抗原刺激后产生。该因子能维持一种对小鼠IL3敏感的细胞系（DA2）的生长。此外，HILDA是嗜酸性粒细胞的强效激活剂，对人类骨髓具有促爆发生长活性。在本研究中，通过依次进行连续浓缩、伴刀豆球蛋白A（ConA）亲和层析及差异洗脱（α-D吡喃葡萄糖苷和α-D甘露糖苷）、高效液相色谱（HPLC）凝胶过滤和反相HPLC，从T细胞克隆上清液中纯化得到了均一的HILDA。在还原或非还原条件下，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示该纯物质为一种38-kd的糖蛋白。从对应于38-kd条带的SDS-PAGE凝胶切片中可恢复生物活性。根据DA-2细胞系的特异性及所描述的生化特性，我们得出结论，这种淋巴因子不同于人类T淋巴细胞产生的其他已知因子。
