Moreau J F, Bonneville M, Godard A, Gascan H, Gruart V, Moore M A, Soulillou J P
J Immunol. 1987 Jun 1;138(11):3844-9.
It has long been suggested that eosinophil response observed in certain immunological reactions depends on the release of soluble products from sensitized lymphocytes when exposed to the challenging antigen. We were able to show that alloreactive T cell clones (ATLC) obtained from human rejected kidney produced, when stimulated with specific antigen (kidney donor-B lymphoblastoid cell line) and interleukin 2, a factor triggering the proliferation of a subline (DA-2) of the interleukin 3 sensitive DA-1 murine cell line. The biochemical features of this factor called HILDA (human interleukin DA) and the DA-2 nonresponsiveness to several human T cell lymphokines and cytokines lead us to the conclusion that this 41,000 m.w. glycoprotein could not be likened to already known T cell lymphokines. Highly purified HILDA turned out to be a potent chemoattractant and activator of, respectively, mouse and human eosinophils. It also displayed burst-promoting activity on human marrow.
长期以来，人们一直认为在某些免疫反应中观察到的嗜酸性粒细胞反应取决于致敏淋巴细胞在接触激发抗原时释放的可溶性产物。我们能够证明，从人移植排斥肾中获得的同种异体反应性T细胞克隆（ATLC），在用特异性抗原（肾供体-B淋巴母细胞系）和白细胞介素2刺激时，会产生一种因子，该因子能触发白细胞介素3敏感的DA-1小鼠细胞系的一个亚系（DA-2）的增殖。这种被称为HILDA（人白细胞介素DA）的因子的生化特性以及DA-2对几种人T细胞淋巴因子和细胞因子的无反应性使我们得出结论，这种分子量为41,000的糖蛋白不能与已知的T细胞淋巴因子相类比。高度纯化的HILDA被证明分别是小鼠和人嗜酸性粒细胞的有效趋化剂和激活剂。它对人骨髓也显示出促爆发活性。
