Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, 769008, Odisha, India.
Fish Health Management Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Microbiol Immunol. 2019 Sep;63(9):379-391. doi: 10.1111/1348-0421.12728. Epub 2019 Aug 25.
The immune system with large number of molecules protects the host against a plethora of continuously evolving microbes. Major histocompatibility complex (MHC) molecules serve as cardinal elements of the adaptive immune system responsible for the activation of the adaptive immunity in the host. The present study reports MHCI molecule in freshwater carp, Catla catla, and its differential expression in immunologically relevant tissues post-infection with Gram-negative and Gram-positive bacteria. The MHCI sequence of C. catla had 502 bp nucleotides encoding putative 146 amino acids. The phylogenetic analysis exhibited its evolutionary conservation within the Cyprinidae family and formed a different clade with the higher vertebrates. Simultaneously, CXCR3 and CXCR4 chemokines were cloned and characterized for their expression in infected tissues. Analysis of immunologically relevant tissues of the infected fish exhibited an increase of MHCI gene expression and the down-regulation of CXCR3 and CXCR4 chemokines, indicating a tricky interaction between the innate and adaptive immune system. It was found that intestine, skin and spleen played a crucial role in the contribution of the defense activity which instigated the self-immunity. These immune activities can provide useful information to understand the interaction of self and non-self- immune system in freshwater fish, Catla catla.
免疫系统拥有大量的分子，可以保护宿主免受大量不断进化的微生物的侵害。主要组织相容性复合体 (MHC) 分子是适应性免疫系统的关键组成部分，负责宿主适应性免疫的激活。本研究报告了淡水鲤鱼（Catla catla）中的 MHC I 分子及其在革兰氏阴性和阳性细菌感染后免疫相关组织中的差异表达。C. catla 的 MHC I 序列有 502bp 核苷酸，编码 146 个假定氨基酸。系统发育分析显示，它在鲤科鱼类中具有进化保守性，并与高等脊椎动物形成了不同的分支。同时，克隆并表征了 CXCR3 和 CXCR4 趋化因子，以研究其在感染组织中的表达。对感染鱼类的免疫相关组织进行分析表明，MHC I 基因表达增加，而 CXCR3 和 CXCR4 趋化因子下调，表明先天免疫和适应性免疫系统之间存在复杂的相互作用。研究发现，肠道、皮肤和脾脏在引发自身免疫的防御活动中起着至关重要的作用。这些免疫活动可以为了解淡水鱼类 Catla catla 中自身和非自身免疫系统的相互作用提供有用信息。
