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长链非编码 RNA HCG11 通过与 miR-496/CPEB3 轴合作调节胶质瘤进展。

Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis.

机构信息

Department of Radiology, Division of PET/CT, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

出版信息

Cell Prolif. 2019 Sep;52(5):e12615. doi: 10.1111/cpr.12615. Epub 2019 Jul 16.

DOI:10.1111/cpr.12615
PMID:31310044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6797506/
Abstract

OBJECTIVES

It has been widely reported that long non-coding RNAs (lncRNAs) can participate in multiple biological processes of human cancers. lncRNA HLA complex group 11 (HCG11) has been reported in human cancers as a tumour suppressor. This study focused on investigating the function and mechanism of HCG11 in glioma.

MATERIALS AND METHODS

Based on The Cancer Genome Atlas (TCGA) data set and qRT-PCR analysis, the expression pattern of HCG11 was identified in glioma samples. The mechanism associated with HCG11 downregulation was determined by mechanism experiments. Gain-of-function assays were conducted for the identification of HCG11 function in glioma progression. Mechanism investigation based on the luciferase reporter assay, RIP assay and pull-down assay was used to explore the downstream molecular mechanism of HCG11. The role of molecular pathway in the progression of glioma was analysed in accordance with the rescue assays.

RESULTS

HCG11 was expressed at low level in glioma samples compared with normal samples. FOXP1 could bind with HCG11 and transcriptionally inactivated HCG11. Overexpression of HCG11 efficiently suppressed cell proliferation, induced cell cycle arrest and promoted cell apoptosis. HCG11 was predominantly enriched in the cytoplasm of glioma cells and acted as a competing endogenous RNAs (ceRNAs) by sponging micro-496 to upregulate cytoplasmic polyadenylation element binding protein 3 (CPEB3). CEPB3 and miR-496 involved in HCG11-mediated glioma progression.

CONCLUSIONS

HCG11 inhibited glioma progression by regulating miR-496/CPEB3 axis.

摘要

目的

大量研究表明长链非编码 RNA（lncRNA）可参与人类癌症的多种生物学过程。lncRNA HLA 复合体基因 11（HCG11）在人类癌症中作为抑癌基因发挥作用。本研究旨在探讨 HCG11 在神经胶质瘤中的作用及机制。

材料和方法

基于癌症基因组图谱（TCGA）数据集和 qRT-PCR 分析，鉴定了 HCG11 在神经胶质瘤样本中的表达模式。通过机制实验确定与 HCG11 下调相关的机制。进行功能获得实验以确定 HCG11 在神经胶质瘤进展中的作用。基于荧光素酶报告实验、RIP 实验和下拉实验的机制研究用于探索 HCG11 的下游分子机制。根据挽救实验分析分子通路在神经胶质瘤进展中的作用。

结果

与正常样本相比，HCG11 在神经胶质瘤样本中表达水平较低。FOXP1 可与 HCG11 结合并转录失活 HCG11。HCG11 的过表达可有效抑制细胞增殖，诱导细胞周期停滞并促进细胞凋亡。HCG11 主要富集在神经胶质瘤细胞的细胞质中，并通过海绵吸附 micro-496 作为竞争性内源 RNA（ceRNA）来上调细胞质多聚腺苷酸化元件结合蛋白 3（CPEB3）。CEPB3 和 miR-496 参与了 HCG11 介导的神经胶质瘤进展。

结论

HCG11 通过调节 miR-496/CPEB3 轴抑制神经胶质瘤的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ca2/6797506/16645209e830/CPR-52-e12615-g008.jpg
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本文引用的文献

1
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Cell Physiol Biochem. 2018;51(3):1364-1375. doi: 10.1159/000495554. Epub 2018 Nov 27.
2
Knockdown of Long Noncoding RNA HOXA-AS2 Suppresses Chemoresistance of Acute Myeloid Leukemia via the miR-520c-3p/S100A4 Axis.长链非编码RNA HOXA-AS2的敲低通过miR-520c-3p/S100A4轴抑制急性髓系白血病的化疗耐药性。
Cell Physiol Biochem. 2018;51(2):886-896. doi: 10.1159/000495387. Epub 2018 Nov 22.
3
LncRNA XIST/miR-34a axis modulates the cell proliferation and tumor growth of thyroid cancer through MET-PI3K-AKT signaling.
Long Non-Coding RNA and as a Promising Diagnostic and Prognostic Biomarker in Glioblastoma Multiforme.
长链非编码RNA作为多形性胶质母细胞瘤中一种有前景的诊断和预后生物标志物。
Iran J Public Health. 2024 Sep;53(9):2090-2102. doi: 10.18502/ijph.v53i9.16462.
4
LncRNA NUTM2A-AS1 silencing inhibits glioma via miR-376a-3p/YAP1 axis.长链非编码RNA NUTM2A-AS1沉默通过miR-376a-3p/YAP1轴抑制胶质瘤。
Cell Div. 2024 May 10;19(1):17. doi: 10.1186/s13008-024-00122-0.
5
Insights into the Role of LncRNAs and miRNAs in Glioma Progression and Their Potential as Novel Therapeutic Targets.长链非编码RNA和微小RNA在胶质瘤进展中的作用及其作为新型治疗靶点的潜力的见解
Cancers (Basel). 2023 Jun 22;15(13):3298. doi: 10.3390/cancers15133298.
6
The role of lncRNA-mediated ceRNA regulatory networks in pancreatic cancer.长链非编码RNA介导的竞争性内源RNA调控网络在胰腺癌中的作用
Cell Death Discov. 2022 Jun 14;8(1):287. doi: 10.1038/s41420-022-01061-x.
7
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8
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Bioengineered. 2022 Jan;13(1):140-154. doi: 10.1080/21655979.2021.2010367.
9
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10
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Exp Ther Med. 2022 Jan;23(1):28. doi: 10.3892/etm.2021.10950. Epub 2021 Nov 8.
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J Exp Clin Cancer Res. 2018 Nov 21;37(1):279. doi: 10.1186/s13046-018-0950-9.
4
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Cell Death Dis. 2018 Nov 19;9(12):1149. doi: 10.1038/s41419-018-1187-4.
5
Long non-coding RNA DANCR promotes malignant phenotypes of bladder cancer cells by modulating the miR-149/MSI2 axis as a ceRNA.长链非编码 RNA DANCR 通过作为 ceRNA 调节 miR-149/MSI2 轴促进膀胱癌细胞的恶性表型。
J Exp Clin Cancer Res. 2018 Nov 12;37(1):273. doi: 10.1186/s13046-018-0921-1.
6
Long Noncoding RNA HOXA-AS2 Promotes Papillary Thyroid Cancer Progression by Regulating miR-520c-3p/S100A4 Pathway.长链非编码RNA HOXA-AS2通过调控miR-520c-3p/S100A4通路促进甲状腺乳头状癌进展。
Cell Physiol Biochem. 2018;50(5):1659-1672. doi: 10.1159/000494786. Epub 2018 Nov 1.
7
Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.长链非编码 RNA AFAP1-AS1 通过调控 Rho/Rac 通路作为 miR-423-5p 的竞争性内源性 RNA 促进鼻咽癌转移。
J Exp Clin Cancer Res. 2018 Oct 16;37(1):253. doi: 10.1186/s13046-018-0918-9.
8
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Cell Physiol Biochem. 2018;45(5):1975-1985. doi: 10.1159/000487974. Epub 2018 Mar 6.
9
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Biochim Biophys Acta Mol Basis Dis. 2018 May;1864(5 Pt A):1783-1794. doi: 10.1016/j.bbadis.2018.02.022. Epub 2018 Mar 1.
10
Long Noncoding RNA H19 Promotes Proliferation and Invasion in Human Glioma Cells by Downregulating miR-152.长链非编码RNA H19通过下调miR-152促进人胶质瘤细胞的增殖和侵袭。
Oncol Res. 2018 Oct 17;26(9):1419-1428. doi: 10.3727/096504018X15178768577951. Epub 2018 Feb 8.