Longo Cameron M, Musah Rabi A
Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave, Albany, NY, 12222.
J Forensic Sci. 2020 Jan;65(1):61-66. doi: 10.1111/1556-4029.14134. Epub 2019 Jul 16.
Unregulated cacti from the genus Echinopsis are used recreationally as mescaline-containing alternatives to the outlawed peyote. Echinopsis-derived plant materials appear in a variety of nondescript forms, making rapid assessment of whether they are mescaline-containing materials or simply innocuous plant-derived food products, very challenging. Reported here is a DART-HRMS approach for the rapid detection of mescaline in whole plant material and a validated method for the quantification of mescaline in cactus tissue, using mescaline-d as the internal standard. Calibration curves exhibited R values of ≥0.995, and the method exhibited a LLOQ and a linear range of 1 ppm and 1-100 ppm, respectively. Application of the method to commercially available Echinopsis spp. yielded results consistent with previous studies performed by GC- and LC-MS, with mescaline levels of <2% dry weight in all cases. Therefore, DART-HRMS is a suitable technique for the rapid screening of mescaline and its subsequent quantification within complex plant-derived matrices.
来自仙人球属的未受管制的仙人掌被作为含三甲氧苯乙胺的替代品用于娱乐，以替代被取缔的佩奥特仙人掌。源自仙人球属的植物材料呈现出各种难以描述的形态，这使得快速评估它们是含三甲氧苯乙胺的材料还是仅仅是无害的植物源性食品极具挑战性。本文报道了一种用于在全植物材料中快速检测三甲氧苯乙胺的DART-HRMS方法，以及一种以氘代三甲氧苯乙胺为内标物对仙人掌组织中三甲氧苯乙胺进行定量的经过验证的方法。校准曲线的R值≥0.995，该方法的定量下限和线性范围分别为1 ppm和1 - 100 ppm。将该方法应用于市售仙人球属植物，所得结果与先前通过气相色谱和液相色谱-质谱法进行的研究一致，所有情况下三甲氧苯乙胺含量均<2%干重。因此，DART-HRMS是一种适用于在复杂植物源性基质中快速筛查三甲氧苯乙胺及其后续定量的技术。
