Department of Chemistry, University at Albany, State University of New York (SUNY), 1400 Washington Avenue, Albany, NY, 12222, USA.
Rapid Commun Mass Spectrom. 2019 Dec 30;33(24):1915-1925. doi: 10.1002/rcm.8532.
Classified by the UNODC as a top 20 plant of concern, Piper methysticum (also known as Kava) is being increasingly abused recreationally for its mind-altering effects. It is of significant forensic relevance to establish methods to rapidly identifyand quantify psychoactive compounds, especially those yet to be scheduled ascontrolled substances and which have exhibited various noteworthy health concerns.
Direct analysis in real time high-resolution mass spectrometry (DART-HRMS) demonstrated the ability to detect a range of kavalactones in Pipermethysticum derived products and plant material with no sample preparation. Inaddition, a validated method using calibration curves developed with a deuteratedinternal standard was used for the quantification of the psychoactive moleculeyangonin in various products.
DART-HRMS detected the protonated masses of six major kavalactonesand three flavokavains in 18 commercial Kava products. A method consistent withFDA validation guidelines was established for the quantification of yangonin in thevarious complex matrices. Implementation of this method, with an LLOQ of 5 mg/mL, enabled successful quantification of yangonin in 16 Kava products.Concentrations for solid products ranged from 2.71 to 8.99 mg/g, while that forliquid products ranged from 1.03 to 4.59 mg/mL.
Rapid identification and quantification of psychoactive smallmolecules in plant material can be accomplished using a validated DART-HRMSprotocol. This work illustrates an approach to qualitative and quantitative analysesof a wide variety of complex matrices derived from plants, and demonstrates thatthe commercially available products analyzed are P. methysticum derived and docontain psychoactive yangonin at quantifiable levels.
被 UNODC 列为前 20 种受关注植物之一的 Piper methysticum（也称为卡瓦），由于其改变思维的作用，正越来越多地被娱乐性滥用。快速识别和定量分析精神活性化合物，特别是那些尚未被列为受控物质且具有各种显著健康问题的化合物，具有重要的法医学意义。
实时高分辨率直接分析质谱（DART-HRMS）证明了无需样品制备即可检测源自 Piper methysticum 的产品和植物材料中一系列卡瓦内酯的能力。此外，还使用使用氘代内标物开发的校准曲线建立了经过验证的方法，用于对各种产品中的精神活性分子杨酮进行定量。
DART-HRMS 检测到 18 种商业卡瓦产品中六种主要卡瓦内酯和三种 flavokavains 的质子化质量。建立了一种符合 FDA 验证指南的方法，用于定量各种复杂基质中的杨酮。该方法的实施，LLOQ 为 5 mg/mL，成功定量了 16 种卡瓦产品中的杨酮。固体产品的浓度范围为 2.71 至 8.99 mg/g，而液体产品的浓度范围为 1.03 至 4.59 mg/mL。
使用经过验证的 DART-HRMS 方案可以快速识别和定量植物材料中的精神活性小分子。这项工作说明了一种对源自植物的各种复杂基质进行定性和定量分析的方法，并证明了所分析的商业上可获得的产品是源自 Piper methysticum 的，并且含有可定量的精神活性杨酮。
