靶向获取 f. sp. 毒素缺陷型突变体及其对西瓜枯萎病的影响。
Targeted Acquisition of f. sp. Toxin-Deficient Mutant and Its Effects on Watermelon Wilt.
机构信息
Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences , Nanjing Normal University , Nanjing , Jiangsu Province 210023 , China.
National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences , Chinese Academy of Sciences , Shanghai 200032 , China.
出版信息
J Agric Food Chem. 2019 Aug 7;67(31):8536-8547. doi: 10.1021/acs.jafc.9b02172. Epub 2019 Jul 26.
Watermelon wilt is a common soil-borne disease that has significantly affected its yield. In this study, fusaric acid-deficient mutant designated as ΔFUBT (mutated from f. sp. , FON) was obtained. The ΔFUBT mutant showed significant decrease in fusaric acid production but maintained wild-type characteristics, such as in vitro colony morphology, size, and conidiation. A field pot experiment demonstrated that ΔFUBT could successfully colonize the rhizosphere and the roots of watermelon, leading to significant reduction in FON colonization in the watermelon plant. In addition, ΔFUBT inoculation significantly improved the rhizosphere microenvironment and effectively increased the resistance in watermelon. This study demonstrated that a nonpathogenic mutant (ΔFUBT) could be developed as an effective microbial control agent to alleviate wilt disease in watermelon and increase its yield.
西瓜枯萎病是一种常见的土传病害，严重影响了其产量。本研究获得了一株缺乏葫芦素酸的突变体，命名为ΔFUBT（来源于 f. sp., FON）。该突变体ΔFUBT 中葫芦素酸的产量明显下降，但仍保持野生型的特征，如体外菌落形态、大小和产孢。田间盆栽试验表明，ΔFUBT 可成功定殖西瓜根际和根系，导致西瓜植株中 FON 定殖的显著减少。此外，ΔFUBT 的接种显著改善了根际微环境，有效增强了西瓜的抗性。本研究表明，非致病性突变体（ΔFUBT）可作为一种有效的微生物防治剂，用于缓解西瓜枯萎病并提高其产量。
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