Laboratório de Tripanosomatídeos, Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Rua Piauí, Bloco 2B Sala 200 Campus Umuarama, 38400-902, Uberlândia, MG, Brazil.
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina, São Paulo - São Paulo, Brazil.
Microb Pathog. 2019 Oct;135:103618. doi: 10.1016/j.micpath.2019.103618. Epub 2019 Jul 13.
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T. cruzi infection remains unknown. Here, we reported the role of P21 in mice harboring late T. cruzi infection. Treatment with recombinant P21 protein (rP21) reduced parasite load and angiogenesis, and induced fibrosis in the cardiac tissue of infected mice. In addition, rP21 reduced the growth of epimastigotes, inhibited intracellular replication of amastigotes and modulated the parasite cell cycle. Our data suggest that P21 controls parasite replication in the host, supporting the survival of both parasite and host.
P21 是由克氏锥虫（T. cruzi）分泌的一种蛋白质。先前的研究表明，P21 具有多种生物学活性，如诱导吞噬作用、白细胞趋化作用和抑制血管生成。然而，P21 在克氏锥虫感染中的活性尚不清楚。在这里，我们报道了 P21 在感染晚期克氏锥虫的小鼠中的作用。用重组 P21 蛋白（rP21）处理可降低寄生虫载量和血管生成，并诱导感染小鼠心脏组织纤维化。此外，rP21 还降低了滋养体的生长，抑制了内阿米巴滋养体的复制，并调节了寄生虫的细胞周期。我们的数据表明，P21 控制着宿主中的寄生虫复制，支持寄生虫和宿主的生存。
