Faculdade de Medicina de Petrópolis (FMP-FASE), Petrópolis, Brazil.
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Front Immunol. 2018 Apr 13;9:671. doi: 10.3389/fimmu.2018.00671. eCollection 2018.
Few studies investigate the major protein antigens targeted by the antibody diversity of infected mice with . To detect global IgG antibody specificities, sera from infected mice were immunoblotted against whole extracts. By proteomic analysis, we were able to identify the most immunogenic proteins. We identified three major antigens as pyruvate phosphate dikinase, Hsp-85, and β-tubulin. The major protein band recognized by host IgG was β-tubulin. The β-tubulin gene was cloned, expressed in , and recombinant β-tubulin was obtained. Infection increased IgG reactivity against recombinant β-tubulin. A single immunization of mice with recombinant β-tubulin increased specific IgG reactivity and induced protection against infection. These results indicate that repertoire analysis is a valid approach to identify antigens for vaccines against Chagas disease.
目前，针对感染 的小鼠体内抗体多样性所针对的主要蛋白抗原，仅有少数研究进行了调查。为了检测全 IgG 抗体的特异性，我们用整个 提取物对感染小鼠的血清进行免疫印迹分析。通过蛋白质组学分析，我们能够鉴定出最具免疫原性的蛋白质。我们鉴定出三种主要的抗原，即丙酮酸磷酸二激酶、Hsp-85 和 β-微管蛋白。宿主 IgG 识别的主要蛋白带为 β-微管蛋白。克隆了 β-微管蛋白基因，在 中表达，并获得了重组 β-微管蛋白。感染增加了针对重组 β-微管蛋白的 IgG 反应性。用重组 β-微管蛋白对小鼠进行单次免疫接种，增加了特异性 IgG 反应性，并诱导了对 感染的保护。这些结果表明，库分析是一种有效的方法，可以鉴定出对抗恰加斯病疫苗的抗原。
