Biotechnology Research Center, and Student Research Committee, Tabriz University of Medical Science, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 1;1125:121701. doi: 10.1016/j.jchromb.2019.06.028. Epub 2019 Jun 29.
A nano graphene oxide sol-gel composite (NGO/sol-gel) applied as a coating of a capillary glass tube stir bar to develop a novel stir bar sorptive extraction (SBSE) method for simultaneous extraction of amphetamine (AMP) and methamphetamine (MET) from biological urine sample. Lab-synthesized NGO was applied with methyltrimethoxysilane (MTMOS) and Tetraethoxysilane (TEOS) as sol-gel precursor. NGO/sol-gel was deposited on the surface of a capillary glass tube to prepare stir bar sorptive extraction adsorbent by a simple and fast method. The scanning electron micrograph images showed a three dimensional structure of lab-made device suitable for SBSE method for simultaneous extraction of AMP and MET. Effective extraction parameters were investigated. Through studied suitable extraction conditions, satisfactory linearity was achieved in the concentration range of 50-2000 ngmL for AMP and 40-2500 ngmL for MET. The relative recovery of the analytes were 99.5 and 99.7% for AMP and MET, respectively for positive urine samples were studied by novel introduced method. The results cleared that NGO/sol-gel composite could be used as practical method in laboratories as an efficient SBSE adsorbent for drugs determination in urine matrix.
一种纳米氧化石墨烯溶胶-凝胶复合材料（NGO/sol-gel）被应用于涂覆在毛细管玻璃搅拌棒上，开发了一种新型的搅拌棒吸附萃取（SBSE）方法，用于同时从生物尿液样品中萃取苯丙胺（AMP）和甲基苯丙胺（MET）。实验室合成的 NGO 与甲基三甲氧基硅烷（MTMOS）和四乙氧基硅烷（TEOS）一起作为溶胶-凝胶前体应用。NGO/sol-gel 通过一种简单快速的方法沉积在毛细管玻璃管的表面上，制备用于 SBSE 方法的搅拌棒吸附萃取吸附剂。扫描电子显微镜图像显示了适合 SBSE 方法同时萃取 AMP 和 MET 的实验室制造装置的三维结构。研究了有效的萃取参数。通过研究合适的萃取条件，在 AMP 的浓度范围为 50-2000 ngmL 和 MET 的浓度范围为 40-2500 ngmL 时，实现了令人满意的线性。通过新型引入的方法研究了阳性尿液样品中分析物的相对回收率，AMP 和 MET 的相对回收率分别为 99.5%和 99.7%。结果表明，NGO/sol-gel 复合材料可用作实验室中的实用方法，作为尿液基质中药物测定的高效 SBSE 吸附剂。
