1 Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
2 The First Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu, China.
Technol Cancer Res Treat. 2019 Jan 1;18:1533033819846638. doi: 10.1177/1533033819846638.
In this study, we aimed to clarify the effects of long noncoding ribonucleic acid prostrate androgen-regulated transcript-1 on bladder cancer cell proliferation and apoptosis.
Microarrays were implemented to investigate the long noncoding ribonucleic acid expression profiles in bladder cancer tissue (N = 9) and in noncancer bladder tissue (N = 5). Relative prostrate androgen-regulated transcript-1 expression levels in tissue samples or cell lines were detected by real-time quantitative reverse transcription-polymerase chain reaction. Prostrate androgen-regulated transcript-1 expression was enhanced by the transfection of pcDNA3.1-prostrate androgen-regulated transcript-1 and downregulated by the infection with pcMV-sh prostrate androgen-regulated transcript-1. Additionally, cell proliferation and apoptosis were measured by the cell counting kit-8 assay and flow cytometry, respectively. Cell invasion was determined by a Transwell assay.
Prostrate androgen-regulated transcript-1 expression was upregulated in bladder cancer tissues compared to adjacent nontumor tissues. Furthermore, prostrate androgen-regulated transcript-1 levels were successfully upregulated by pcDNA3.1-prostrate androgen-regulated transcript-1 and depleted by pCMV-sh prostrate androgen-regulated transcript-1 in bladder cancer cell lines (5637, T24). Enhanced prostrate androgen-regulated transcript-1 expression promoted cell proliferation and invasion and inhibited cell apoptosis. However, knockdown of prostrate androgen-regulated transcript-1 expression inhibited cell proliferation and invasion and induced cell apoptosis.
In summary, these data suggest that the knockdown of prostrate androgen-regulated transcript-1 represents a tumor suppressor player in bladder cancer and contributes to the inhibition of tumor proliferation, the promotion of cell apoptosis, and the suppression of cell invasion. Prostrate androgen-regulated transcript-1 may function as a new prognostic biomarker and as a feasible therapeutic target for patients with bladder cancer.
本研究旨在阐明长链非编码 RNA 前列腺雄激素调控转录本 1 对膀胱癌细胞增殖和凋亡的影响。
采用微阵列技术检测 9 例膀胱癌组织和 5 例非癌膀胱组织中的长链非编码 RNA 表达谱。实时定量逆转录聚合酶链反应检测组织样本或细胞系中相对前列腺雄激素调控转录本 1 的表达水平。通过 pcDNA3.1-前列腺雄激素调控转录本 1 转染增强前列腺雄激素调控转录本 1 的表达，通过 pcMV-sh 前列腺雄激素调控转录本 1 感染下调前列腺雄激素调控转录本 1 的表达。此外，通过细胞计数试剂盒-8 检测和流式细胞术分别检测细胞增殖和凋亡。通过 Transwell 测定法检测细胞侵袭。
与相邻非肿瘤组织相比，膀胱癌组织中前列腺雄激素调控转录本 1 的表达上调。此外，在膀胱癌细胞系（5637、T24）中，pcDNA3.1-前列腺雄激素调控转录本 1 成功上调前列腺雄激素调控转录本 1 水平，而 pCMV-sh 前列腺雄激素调控转录本 1 下调前列腺雄激素调控转录本 1 表达。增强的前列腺雄激素调控转录本 1 表达促进细胞增殖和侵袭，抑制细胞凋亡。然而，下调前列腺雄激素调控转录本 1 表达抑制细胞增殖和侵袭，诱导细胞凋亡。
综上所述，这些数据表明，下调前列腺雄激素调控转录本 1 代表膀胱癌中的肿瘤抑制因子，有助于抑制肿瘤增殖、促进细胞凋亡和抑制细胞侵袭。前列腺雄激素调控转录本 1 可能作为膀胱癌的新预后生物标志物和可行的治疗靶点。
