In-house rapid colorimetric method for detection of colistin resistance in : A significant impact on resistance rates.
作者信息
Rodriguez Carlos Hernán, Maza Jonathan, Tamarin Samanta, Nastro Marcela, Vay Carlos, Famiglietti Angela
机构信息
Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA, Argentina.
出版信息
J Chemother. 2019 Nov-Dec;31(7-8):432-435. doi: 10.1080/1120009X.2019.1637557. Epub 2019 Jul 17.
A rapid colorimetric method, the Andrade Screening Antimicrobial Test (ASAT) was evaluated to detect colistin resistance in clinical isolates. The sensitivity and specificity were 90.7% and 100%, respectively. In 10/26 isolates the automatized method failed to detect the resistance, whereas the ASAT detected it accurately. Most of these isolates showed COL MIC values in the range 4-8 µg mL and carried -1. As regards COL- resistant isolates, discrepancies between the Phoenix system and the ASAT were observed only in 3/44 isolates, most of them carried the gene and showed COL MIC values >16 µg mL.
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