Alamian Saeed, Dadar Maryam
Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Prev Vet Med. 2019 Aug 1;169:104708. doi: 10.1016/j.prevetmed.2019.104708. Epub 2019 Jun 17.
Brucella spp. commonly infect humans in various regions worldwide. Human brucellosis mainly spreads through the consumption of contaminated raw dairy products and meat from domestic livestock (water buffalo, goats, sheep, cattle, pigs and camels). In this regard, the origin and routes of transmission of this bacterium should be carefully determined in order to control the source of infection. This study aimed to evaluate the rate of Brucella spp. contamination of camel milk samples sent for analysis to the national brucellosis laboratory during 2018 in Iran. For this purpose, 96 milk samples from 96 dairy camel herds were randomly collected from two provinces and investigated for the presence of Brucella spp contaminations by both bacterial culture method and polymerase chain reaction (PCR). No clinical manifestation of brucellosis was reported in camels from which milk samples were collected. Using the culture method, three milk samples (3%) originating from two camels of Isfahan province (4%) and one camel from the Semnan province (2%), were contaminated with Brucella abortus. According to PCR analyses, B. abortus gene was detected in 14 (14.5%) milk samples, including 9 and 5 samples from Isfahan (18%) and Semnan (11%) province, respectively. PCR method revealed significant differences (p = 0.02) in the level of contamination with B. abortus between milk samples collected from two regions. These results represent the first report regarding the isolation of B. abortus from raw camel milk in Iran and highlight the importance to screen apparent healthy camels. Therefore, the consumption of raw camel milk may contribute to the spread of human brucellosis in endemic regions.
布鲁氏菌属在全球多个地区普遍感染人类。人类布鲁氏菌病主要通过食用受污染的生鲜乳制品以及来自家畜（水牛、山羊、绵羊、牛、猪和骆驼）的肉类传播。在这方面，应仔细确定这种细菌的起源和传播途径，以控制传染源。本研究旨在评估2018年期间送往伊朗国家布鲁氏菌病实验室进行分析的骆驼奶样本中布鲁氏菌属的污染率。为此，从两个省份随机收集了来自96个奶用骆驼群的96份奶样，并通过细菌培养法和聚合酶链反应（PCR）检测布鲁氏菌属污染情况。采集奶样的骆驼均未报告有布鲁氏菌病的临床表现。采用培养法，来自伊斯法罕省两头骆驼（4%）和塞姆南省一头骆驼（2%）的三份奶样（3%）被流产布鲁氏菌污染。根据PCR分析，在14份（14.5%）奶样中检测到流产布鲁氏菌基因，其中分别有9份和5份来自伊斯法罕省（18%）和塞姆南省（11%）。PCR方法显示，从两个地区采集的奶样中流产布鲁氏菌的污染水平存在显著差异（p = 0.02）。这些结果代表了伊朗首次从生鲜骆驼奶中分离出流产布鲁氏菌的报告，并突出了筛查表面健康骆驼的重要性。因此，食用生鲜骆驼奶可能会导致布鲁氏菌病在流行地区的传播。
