蓝藻质粒pDU1在鱼腥藻M-131菌株中复制所需区域的鉴定。

Identification of the region of cyanobacterial plasmid pDU1 necessary for replication in Anabaena sp. strain M-131.

作者信息

Schmetterer G, Wolk C P

机构信息

MSU-DOE Plant Research Laboratory, East Lansing 48824.

出版信息

Gene. 1988;62(1):101-9. doi: 10.1016/0378-1119(88)90583-5.

DOI:10.1016/0378-1119(88)90583-5

PMID:3131190

Abstract

Shuttle vectors based on plasmid pDU1 from Nostoc sp. strain PCC7524 are able to replicate both in Escherichia coli and in strains of Anabaena and Nostoc spp. Derivatives partially deleted in the pDU1 portion were tested for their ability to replicate in Anabaena sp. strain M-131. Plasmid pRL6HE containing a 1.75-kb HindIII-ScaI fragment of pDU1 replicated stably in that cyanobacterium and also in Anabaena sp. strain PCC7120. Plasmid pRL6HC, containing an even smaller HpaI-ScaI fragment (1.3 kb) replicated in Anabaena sp. strain M-131 but not in Anabaena sp. strain PCC7120. Similarly, when the 1.75-kb fragment of pDU1 was transferred from pRL6HE to another vector (pRL40 delta), the resulting plasmids replicated in Anabaena sp. strain M-131 but not in Anabaena sp. strain PCC7120.

摘要

基于来自念珠藻属菌株PCC7524的质粒pDU1构建的穿梭载体能够在大肠杆菌以及鱼腥藻属和念珠藻属的菌株中复制。对在pDU1部分进行了部分缺失的衍生物在鱼腥藻属菌株M-131中的复制能力进行了测试。含有pDU1的1.75 kb HindIII - ScaI片段的质粒pRL6HE在该蓝细菌以及鱼腥藻属菌株PCC7120中稳定复制。含有甚至更小的HpaI - ScaI片段（1.3 kb）的质粒pRL6HC在鱼腥藻属菌株M-131中复制，但不在鱼腥藻属菌株PCC7120中复制。同样，当pDU1的1.75 kb片段从pRL6HE转移到另一个载体（pRL40 delta）时，所得质粒在鱼腥藻属菌株M-131中复制，但不在鱼腥藻属菌株PCC7120中复制。