Lyu Weiyuan, Yu Mengchao, Qu Haijun, Yu Ziqing, Du Wenbin, Shen Feng
School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Biomicrofluidics. 2019 Jul 12;13(4):041502. doi: 10.1063/1.5109270. eCollection 2019 Jul.
Slip-driven microfluidic devices can manipulate fluid by the relative movement of microfluidic plates that are in close contact. Since the demonstration of the first SlipChip device, many slip-driven microfluidic devices with different form factors have been developed, including SlipPAD, SlipDisc, sliding stripe, and volumetric bar chart chip. Slip-driven microfluidic devices can be fabricated from glass, quartz, polydimethylsiloxane, paper, and plastic with various fabrication methods: etching, casting, wax printing, laser cutting, micromilling, injection molding, etc. The slipping operation of the devices can be performed manually, by a micrometer with a base station, or autonomously, by a clockwork mechanism. A variety of readout methods other than fluorescence microscopy have been demonstrated, including both fluorescence detection and colorimetric detection by mobile phones, direct visual detection, and real-time fluorescence imaging. This review will focus on slip-driven microfluidic devices for nucleic acid analysis, including multiplex nucleic acid detection, digital nucleic acid quantification, real-time nucleic acid amplification, and sample-in-answer-out nucleic acid analysis. Slip-driven microfluidic devices present promising approaches for both life science research and clinical molecular diagnostics.
滑动驱动的微流控装置可通过紧密接触的微流控板的相对运动来操控流体。自首个SlipChip装置问世以来，已研发出许多具有不同外形尺寸的滑动驱动微流控装置，包括SlipPAD、SlipDisc、滑动条纹和体积柱状图芯片。滑动驱动的微流控装置可用玻璃、石英、聚二甲基硅氧烷、纸张和塑料通过各种制造方法制成：蚀刻、铸造、蜡印、激光切割、微铣削、注塑成型等。装置的滑动操作可手动进行，通过带有基站的千分尺进行，或自主进行，通过发条机构进行。除荧光显微镜外，还展示了多种读出方法，包括通过手机进行荧光检测和比色检测、直接视觉检测和实时荧光成像。本综述将聚焦于用于核酸分析的滑动驱动微流控装置，包括多重核酸检测、数字核酸定量、实时核酸扩增和样本进答案出核酸分析。滑动驱动的微流控装置为生命科学研究和临床分子诊断提供了有前景的方法。
