Blomgren H, Hammarström S, Wasserman J
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
Int Arch Allergy Appl Immunol. 1988;86(1):62-8. doi: 10.1159/000234607.
Previously we reported that inhibitors of the enzyme cyclo-oxygenase, which reduce biosynthesis of prostaglandins, may enhance mitogenic responses of human blood lymphoid cells, whereas only marginal effects were observed with 5,8,11-eicosatriynoic acid (ETI), which inhibits 12-lipoxygenase and leukotriene biosynthesis. However, sharply enhanced mitogen responses were observed when lymphoid cells were cultured with both a cyclo-oxygenase inhibitor and ETI, suggesting a synergy between the two drugs. To test whether this is a more general phenomenon occurring between inhibitors of cyclo-oxygenase and lipoxygenase, we have now extended these studies to include the following lipoxygenase inhibitors: cirsiliol; esculetin; 5,8,11,14-eicosatetraynoic acid, and nordihydroguaiaretic acid. The results have shown that any of these drugs combined with a cyclo-oxygenase inhibitor may enhance mitogen responses more than one would expect by summing the effects of the inhibitors tested separately. We conclude that inhibitors of cyclo-oxygenase and lipoxygenase may synergistically enhance mitogen responses of lymphoid cells.
此前我们报道，可减少前列腺素生物合成的环氧化酶抑制剂，可能会增强人血淋巴细胞的促有丝分裂反应，而抑制12-脂氧合酶和白三烯生物合成的5,8,11-二十碳三烯酸（ETI），仅观察到轻微作用。然而，当淋巴细胞与环氧化酶抑制剂和ETI共同培养时，观察到促有丝分裂反应急剧增强，提示这两种药物之间存在协同作用。为了检验这是否是环氧化酶抑制剂和脂氧合酶抑制剂之间发生的更普遍现象，我们现在将这些研究扩展至包括以下脂氧合酶抑制剂：刺鼠李素；七叶亭；5,8,11,14-二十碳四烯酸，以及去甲二氢愈创木酸。结果表明，这些药物中的任何一种与环氧化酶抑制剂联合使用，增强促有丝分裂反应的程度可能超过分别测试抑制剂作用之和。我们得出结论，环氧化酶抑制剂和脂氧合酶抑制剂可能协同增强淋巴细胞的促有丝分裂反应。
