Department of Agronomy and Plant Breeding, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran.
Faculty Member of Research Department of Forests and Rangelands, Kermanshah Agricultural and Natural Resources Research and Education Center, AREEO, Kermanshah, Iran.
Mol Biol Rep. 2019 Oct;46(5):5209-5223. doi: 10.1007/s11033-019-04978-2. Epub 2019 Jul 16.
The genus of Bromus is one of the most important collection of rangeland plants, which are distributed in a wide range of natural areas of Iran. Interspecific relationships were evaluated in 90 accessions of 18 Bromus species based on 15 ISSR and 15 SCoT primers. SCoT markers separated the accessions better than ISSR marker. In addition, there was a high interspecific diversity between surveying germplasm. The sections of Bromus genus completely separated based on DNA molecular markers. SCoT markers could separate the accessions in each species. The primers of SC and SC from SCoT marker and UBC861, UBC857 and UBC844 primers from ISSR marker were identified as the best primers in revealing of genetic diversity between accessions. The sections of Ceratochloa, Genea, Pnigma and Bromus were monophyletic and were placed in one cluster. The section Bromus had a direct relationship with section Genea. In other words, section Ceratochloa has a direct relationship with Pnigma. B. tectorum and B. sericeus. B. sterilis had the most distance with other species in section Genea. B. squarrosus and B. japonicus had the most similarity and B. briziformis with B. danthoniae and B. scoparius with B. rechingeri had a moderate relationship in section Bromus. B. tomentosus and B. persicus had the highest similarity and B. riparius with B. biebersteinii and B. tomentellus with B. inermis had a moderate similarity in section Pnigma.
雀麦属是最重要的草原植物属之一，分布于伊朗广泛的自然区域。本研究基于 15 个 ISSR 和 15 个 SCoT 引物，对 18 种雀麦属 90 个种源进行了种间关系评价。SCoT 标记比 ISSR 标记更好地分离了种源。此外，在调查的种质中存在高度的种间多样性。雀麦属的各个节段完全基于 DNA 分子标记分离。SCoT 标记可以分离每个种的种源。SCoT 标记的 SC 和 SC 引物以及 ISSR 标记的 UBC861、UBC857 和 UBC844 引物被鉴定为揭示种源间遗传多样性的最佳引物。Ceratochloa、Genea、Pnigma 和 Bromus 节段是单系的，被放在一个聚类中。Bromus 节段与 Genea 节段有直接关系。换句话说，Ceratochloa 节段与 Pnigma 节段有直接关系。B. tectorum 和 B. sericeus。B. sterilis 与 Genea 节段中的其他种源距离最远。B. squarrosus 和 B. japonicus 最为相似，B. briziformis 与 B. danthoniae 和 B. scoparius 与 B. rechingeri 关系适中，在 Bromus 节段中。B. tomentosus 和 B. persicus 最为相似，B. riparius 与 B. biebersteinii 和 B. tomentellus 与 B. inermis 关系适中，在 Pnigma 节段中。
