从组织和沉积物提取物中分离有机污染物的高效液相色谱法。

High-performance liquid chromatographic method for isolating organic contaminants from tissue and sediment extracts.

作者信息

Krahn M M, Moore L K, Bogar R G, Wigren C A, Chan S L, Brown D W

机构信息

Environmental Conservation Division, National Oceanic and Atmospheric Administration, Seattle, WA 98112.

出版信息

J Chromatogr. 1988 Mar 11;437(1):161-75. doi: 10.1016/s0021-9673(00)90379-0.

DOI:10.1016/s0021-9673(00)90379-0

PMID:3131379

Abstract

Interest in the assessment of the anthropogenic contamination of the marine environment has accelerated in recent years. Existing methods to analyze environmental samples (e.g., sediments or tissues) for trace amounts of organic contaminants such as aromatic hydrocarbons and chlorinated compounds are tedious and costly. We report a rapid, efficient high-performance liquid chromatography (HPLC) procedure which uses a size-exclusion column to separate the analytes of interest from interfering compounds in the sample matrix. Analytical results from the HPLC method were, in general, comparable to a gravity-column method which had been used for several years. The HPLC method had several other advantages: improved precision; the ability to monitor chromatographic conditions; the potential for automating analyses; and reduced consumption of solvents and other materials.

摘要

近年来，对海洋环境人为污染评估的关注度不断提高。现有的分析环境样品（如沉积物或组织）中痕量有机污染物（如芳烃和氯化化合物）的方法既繁琐又昂贵。我们报告了一种快速、高效的高效液相色谱（HPLC）方法，该方法使用尺寸排阻柱将目标分析物与样品基质中的干扰化合物分离。HPLC方法的分析结果总体上与已使用数年的重力柱法相当。HPLC方法还有其他几个优点：提高了精度；能够监测色谱条件；具有自动化分析的潜力；减少了溶剂和其他材料的消耗。