School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
Faraday Discuss. 2019 Oct 30;219(0):112-127. doi: 10.1039/c9fd00017h.
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.
已制备出对霍乱毒素（CTB）的识别单元具有刺激响应性的受体，其方法是将 CTB 的天然碳水化合物配体 GM1 寡糖的多个拷贝连接到热响应性聚合物支架上。在低于其低临界溶液温度（LCST）下，聚合物以纳摩尔亲和力与 CTB 复合。当加热到 LCST 以上时，聚合物会发生可逆的从线圈到球的转变，使得一部分碳水化合物识别基序无法与 CTB 相互作用。这种热调节的材料对蛋白质的亲合力的降低已被用于从溶液中可逆地捕获 CTB，从而能够方便地从复杂混合物中分离 CTB。
