1Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czechia.
2Department of Paediatrics, University Hospital Motol and Second Faculty of Medicine, Charles University in Prague, Prague, Czechia.
J Med Food. 2019 Aug;22(8):810-816. doi: 10.1089/jmf.2019.0021. Epub 2019 Jul 16.
Current studies indicate a link between the intake of exclusive enteral nutrition (EEN) and the induction of complex changes in the intestinal microbiota, as well as the clinical improvement of Crohn's disease (CD). The first aim of this study was to test the ability of various commensal bacterial strains ( = 19) such as bifidobacteria, lactobacilli, and to grow on three different polymeric EN . Tested EN formulas were found to be suitable growth media for tested commensals. Furthermore, the counts of these bacteria and total counts of anaerobic bacteria in the fecal samples of children with CD ( = 15) before and after 6 weeks of EEN diet administration were determined using cultivation on selective media. The counts of cultivable commensal bacteria in the fecal samples of CD children were not significantly affected by EEN. However, tested bacteria showed some individual shifts in counts before and after EEN therapy. Moreover, cultured bifidobacteria were found to be in reduced counts in CD children. Therefore, the application of bifidogenic prebiotic compounds to EN for CD patients might be considered.
目前的研究表明，肠内营养（EEN）的摄入与肠道微生物群的复杂变化以及克罗恩病（CD）的临床改善之间存在关联。本研究的首要目的是测试双歧杆菌、乳杆菌等各种共生菌株（ = 19）在三种不同的聚合型肠内营养配方上的生长能力。研究发现，测试的肠内营养配方适合于测试共生菌的生长。此外，通过选择性培养基培养，在给予 EEN 饮食治疗前和治疗后 6 周，测定 CD 患儿（ = 15）粪便样本中这些细菌和厌氧菌总数的变化。EEN 对 CD 患儿粪便中可培养共生菌的数量没有显著影响。然而，测试的细菌在 EEN 治疗前后的数量确实存在一些个体变化。此外，还发现 CD 患儿的双歧杆菌数量减少。因此，考虑将双歧杆菌促生的益生元化合物应用于 CD 患者的肠内营养配方中。
