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Isolation and characterization of an immunosuppressive protein from Trichosanthes kirilowii root tubers.

作者信息

Yeung H W, Poon S P, NG T B, Li W W

机构信息

Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.

出版信息

Immunopharmacol Immunotoxicol. 1987;9(1):25-46. doi: 10.3109/08923978709035200.

DOI:10.3109/08923978709035200
PMID:3131412
Abstract

An immunosuppressive protein was isolated from Trichosanthes kirilowii root tubers by a procedure involving acetone fractionation and ion exchange chromatography on CM-Sepharose. Homogeneity of the protein was demonstrated in immunoelectrophoresis, SDS-polyacrylamide gel electrophoresis, gel filtration, high performance liquid chromatography and a single NH2-terminal sequence. The protein had a molecular weight of 26,000, aspartic acid as the NH2-terminal amino acid and no cysteine or carbohydrates in its molecule. It inhibited ConA-induced transformation in lymphocytes isolated from spleens of CBA mice. The protein was also potent in inducing mid-term abortion in mice.

摘要

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