Institute of Clinical Microbiology and Hygiene, University Hospital of Regensburg and University of Regensburg , Regensburg , Germany.
Expert Rev Mol Diagn. 2019 Aug;19(8):659-665. doi: 10.1080/14737159.2019.1645598. Epub 2019 Aug 1.
: Treatment options for infections with carbapenem-resistant Enterobacterales are strongly limited. New antimicrobials are not effective against all types of carbapenemases. Therefore, rapid and reliable antimicrobial susceptibility testing and identification of the resistance mechanism are important. : We assess several methods to determine carbapenemase production of Enterobacterales in culture and discuss the value of the novel automated BD Phoenix CPO Detect (BDPCPO) panel for the detection and classification of carbapenemases. : The meropenem minimum inhibitory concentration (MIC) range used in the BDPCPO panel includes the EUCAST screening breakpoint for the detection of carbapenemase producers. The phenotypic, inhibitor-based assay for detection of carbapenemase activity in the BDPCPO panel displays high sensitivity for carbapenemase detection while its specificity is modest. Therefore, confirmation testing of positive results is warranted. Nevertheless, implementing the BDPCPO panel has the potential to reduce time-to-result for detection and classification of carbapenemase producers.
治疗耐碳青霉烯类肠杆菌科细菌感染的选择方案非常有限。新型抗菌药物并不能有效对抗所有类型的碳青霉烯酶。因此，快速、可靠的抗菌药物敏感性检测和耐药机制的鉴定非常重要。
我们评估了几种方法来确定培养物中肠杆菌科的碳青霉烯酶产生情况，并讨论了新型自动化 BD Phoenix CPO Detect（BDPCPO）检测板在检测和分类碳青霉烯酶方面的价值。
BDPCPO 检测板中使用的美罗培南最小抑菌浓度（MIC）范围包括 EUCAST 用于检测碳青霉烯酶产生菌的筛选临界点。BDPCPO 检测板中用于检测碳青霉烯酶活性的表型、抑制剂基础检测法对碳青霉烯酶检测具有很高的灵敏度，但其特异性一般。因此，阳性结果需要进行确认检测。尽管如此，实施 BDPCPO 检测板有可能减少检测和分类碳青霉烯酶产生菌的结果报告时间。
