Department of Medical Laboratory Science, College of Medicine, University of Lagos, Lagos, Nigeria.
Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University, College of Health Sciences, Sagamu, Nigeria.
Microb Drug Resist. 2019 Dec;25(10):1410-1423. doi: 10.1089/mdr.2018.0305. Epub 2019 Jul 17.
As resistance to the β-lactam class of antibiotics has become a worldwide problem, multidrug-resistant (MDR) human ( = 243) and food animal ( = 211) isolates from Lagos, Nigeria were further tested to characterize β-lactamase-encoding genes and plasmid replicons. Four β-lactamase-encoding genes (, , , and ) were detected using PCR-based replicon typing, 13 and 17 different replicons were identified using a subset of MDR from humans ( = 48) and animals ( = 96), respectively. Replicon types FIB and X2 were detected in equal numbers (2/48; 4.2% each) from human isolates, while type Y (16/96; 16.7%) was the most common type from animals. Only two replicon types, FIB and Y, were detected in both groups; all other types were confined to one group or the other, but not both. Using conjugation, replicon type Y, present in three donors, transferred in all three instances, whereas FIA transferred in 75% (3/4) of the matings. This study showed that β-lactamase genes were prevalent in MDR from both humans and animals in Nigeria and also contained diverse plasmid replicons. As the replicon-associated genes were mobile, they are likely to continue disseminating among and facilitating transfer of associated β-lactamase genes in this region.
由于对β-内酰胺类抗生素的耐药性已成为全球性问题，因此进一步检测了来自尼日利亚拉各斯的多药耐药（MDR）人类（ = 243）和食品动物（ = 211）分离株，以鉴定β-内酰胺酶编码基因和质粒复制子。使用基于 PCR 的复制子分型检测到了 4 种β-内酰胺酶编码基因（、、、和），使用人类（ = 48）和动物（ = 96）的 MDR 的子集鉴定出了 13 和 17 种不同的复制子。在人类分离株中，FIB 和 X2 型复制子的检出率相同（各占 2/48；4.2%），而 Y 型（16/96；16.7%）是动物中最常见的类型。只有两种复制子类型 FIB 和 Y 同时存在于两组中；其他所有类型均局限于一组或另一组，但不在两组中同时存在。通过接合，存在于三个供体中的 Y 型复制子在所有三个实例中均发生转移，而 FIA 仅在 75%（3/4）的交配中转移。本研究表明，β-内酰胺酶基因在尼日利亚的人类和动物的 MDR 中普遍存在，并且还包含多种质粒复制子。由于与复制子相关的基因是可移动的，它们很可能继续在 中传播，并促进该地区相关β-内酰胺酶基因的转移。
