从尼日利亚阿贝奥库塔的家禽养殖场分离出的大肠杆菌的抗菌药物耐药性和毒力基因谱：基于“同一健康”视角的研究
Antimicrobial resistance and virulence gene profiles of Escherichia coli isolated from poultry farms using One Health perspective in Abeokuta, Nigeria.
作者信息
Ajibola A T, de Lagarde M, Ojo O E, Balogun S A, Vanier G, Fairbrother J M, Shittu O B
机构信息
Microbiology Department, College of Biosciences, Federal University of Agriculture, Abeokuta, P.M.B 2240, Abeokuta, 110111, Nigeria.
OMSA Reference Laboratory for Escherichia Coli, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, J2S2M2, Canada.
出版信息
BMC Microbiol. 2025 Jul 16;25(1):440. doi: 10.1186/s12866-025-04135-9.
BACKGROUND
Antimicrobial resistant Extraintestinal pathogenic Escherichia coli (ExPEC) includes uropathogenic E. coli (UPEC), the causative agents of urinary tract infections (UTI), neonatal meningitis E. coli (NMEC), implicated in neonatal meningitis in humans, and avian pathogenic E. coli (APEC). Antimicrobial resistant ExPEC infections are associated with economic losses in poultry worldwide. However, few studies have been done on ExPEC in Nigeria.
METHODS
In order to gain further insight into the presence of antimicrobial resistant ExPEC in poultry and the putative transmission of E. coli isolates from healthy chickens to poultry owners and/or the environment, antimicrobial susceptibility testing (AST) and polymerase chain reaction (PCR) to determine the presence of 5 virulence genes considered as ExPEC predictors were carried out on 71 E. coli isolates from apparently healthy chickens, their handlers and their shared environment, in Abeokuta, Ogun State, Nigeria. In addition, 14 of the 71 isolates were characterized using whole genome sequencing.
RESULTS
Regardless of the source, 70.4% of the isolates were multidrug-resistant (MDR) and 66.2% of the isolates were resistant to fluoroquinolones. Only 3 out of the 71 isolates had at least 2 ExPEC predictor genes and the proportion of antimicrobial resistance (AMR) was higher in isolates with at least 1 ExPEC predictor gene. Thirty different resistance determinants were identified from the 14 whole genome sequenced isolates and most of the sequenced isolates harbored plasmid-mediated resistance genes, including aph(3″)-Ib, aph(6)-Id, floR, sul1, sul2, tet(A), and tet(B). Isolates were very diverse, 11 different serotypes and 12 different sequence types being detected in this study. Twenty different replicons were also found, IncF1B being the most frequently detected. Different ExPEC virulent genes and virulence associated genes (VAGs) for iron acquisition, toxin production, invasion and other virulence determinants were detected.
CONCLUSIONS
Our results suggest that apparently healthy birds carry antimicrobial resistant genes and virulence genes, underlining their possible transmission to humans. The presence of similar ARGs and VAGs in the handlers of birds and their shared environment highlights the importance of using a One Health approach.
背景
耐抗菌药物的肠外致病性大肠杆菌（ExPEC）包括尿路致病性大肠杆菌（UPEC），它是尿路感染（UTI）的病原体；新生儿脑膜炎大肠杆菌（NMEC），与人类新生儿脑膜炎有关；以及禽致病性大肠杆菌（APEC）。耐抗菌药物的ExPEC感染在全球范围内给家禽业带来经济损失。然而，尼日利亚针对ExPEC的研究很少。
方法
为了进一步了解家禽中耐抗菌药物的ExPEC的存在情况以及大肠杆菌分离株从健康鸡只向家禽养殖者和/或环境的假定传播情况，对来自尼日利亚奥贡州阿贝奥库塔的71株大肠杆菌分离株进行了药敏试验（AST）和聚合酶链反应（PCR），以确定5种被视为ExPEC预测指标的毒力基因的存在情况。这些分离株来自看似健康的鸡只、它们的饲养者以及它们共享的环境。此外，对71株分离株中的14株进行了全基因组测序分析。
结果
无论来源如何，70.4%的分离株对多种药物耐药（MDR），66.2%的分离株对氟喹诺酮类耐药。71株分离株中只有3株至少有2个ExPEC预测指标基因，且至少有1个ExPEC预测指标基因的分离株的抗菌药物耐药（AMR）比例更高。从14株全基因组测序的分离株中鉴定出30种不同的耐药决定因素，大多数测序分离株携带质粒介导的耐药基因，包括aph(3″)-Ib、aph(6)-Id、floR、sul1、sul2、tet(A)和tet(B)。分离株非常多样，本研究中检测到11种不同血清型和12种不同序列类型。还发现了20种不同的复制子，其中IncF1B是最常检测到的。检测到不同的ExPEC毒力基因以及与铁摄取、毒素产生、侵袭和其他毒力决定因素相关的毒力相关基因（VAGs）。
结论
我们的结果表明，看似健康的禽类携带抗菌药物耐药基因和毒力基因，这凸显了它们可能向人类传播的风险。禽类饲养者及其共享环境中存在类似的ARGs和VAGs，突出了采用“同一健康”方法的重要性。
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